Sharma Sonia, Sharma Aarjoo, Sodhi Gurleen Kaur, George Nancy, Alarjani Khaloud Mohammed, Mukherjee Arkadeep, Kumar Rath Santosh, Kaur Ramandeep, Dwibedi Vagish
University Institute of Biotechnology, Chandigarh University, Mohali, India.
Department of Microbiology, Panjab University, Chandigarh, India.
Front Microbiol. 2025 Apr 30;16:1546687. doi: 10.3389/fmicb.2025.1546687. eCollection 2025.
Probiotics are live bacteria that provides numerous healthy and beneficial effects to the consumers. The present study aimed to investigate the effect of a probiotic candidate SAS1, in immunoregulation and obesity management.
: This probiotic candidate was isolated from a soil sample collected from a region of fruit waste decomposition. cytotoxicity was assessed using the THP-1 (human leukemia monocytic cell line) cells using MTT assay.
An IC50 value of 47.52 ± 0.18 μg/mL and cell shrinkage were observed along with the release of cellular content of THP-1 cells. The higher production of reactive oxygen species and lesser release of interleukins (IL-4, 5, and 13) are attributed to the antiallergic potential of this strain. Furthermore, in vitro cytotoxicity evaluation using 3T3-L1 cells identified this strain as a promising candidate for anti-obesity treatment. The observed IC50 value was 514.4 ± 0.061 μg/mL.
This extract was shown to have good lipase-inhibiting enzyme activity and was reported to prevent adipogenesis, depicted by increased HDL levels and decreased LDL and triglyceride levels. These results suggested that Staphylococcus epidermidis SAS1 may have therapeutic use in the treatment of obesity and allergies.
益生菌是对消费者有诸多健康有益作用的活细菌。本研究旨在调查候选益生菌SAS1在免疫调节和肥胖管理方面的作用。
该候选益生菌是从水果废弃物分解区域采集的土壤样本中分离得到的。使用MTT法，利用THP-1（人白血病单核细胞系）细胞评估细胞毒性。
观察到THP-1细胞的IC50值为47.52±0.18μg/mL，细胞出现皱缩，并伴有细胞内容物释放。该菌株的抗过敏潜力归因于其活性氧的较高产生量以及白细胞介素（IL-4、5和13）的较少释放。此外，使用3T3-L1细胞进行的体外细胞毒性评估确定该菌株是抗肥胖治疗的有前景候选菌株。观察到的IC50值为514.4±0.061μg/mL。
该提取物显示出良好的脂肪酶抑制酶活性，据报道可预防脂肪生成，表现为高密度脂蛋白水平升高以及低密度脂蛋白和甘油三酯水平降低。这些结果表明表皮葡萄球菌SAS1可能在肥胖症和过敏症治疗中具有治疗用途。
