Cosby S L, McQuaid S, Taylor M J, Bailey M, Rima B K, Martin S J, Allen I V
Queen's University of Belfast, Multiple Sclerosis Laboratory, U.K.
J Gen Virol. 1989 Aug;70 ( Pt 8):2027-36. doi: 10.1099/0022-1317-70-8-2027.
In situ hybridization studies have been carried out on brain samples from eight cases of multiple sclerosis (MS) and 56 non-neurological and neurological controls, using single-stranded 35S-labelled RNA probes prepared against genomic RNA sequences of measles virus, canine distemper virus, rubella virus and simian virus 5. Foci of hybridization were found using probes against the measles virus nucleocapsid protein (N), phosphoprotein and fusion protein gene sequences in two of the MS cases, and also in one control, a case of disseminated cytomegalovirus infection with spinal cord necrosis. This result was confirmed using biotinylated probes prepared against the measles virus N genomic sequence. No hybridization was found in any of the MS or control cases using any of the other viral genome-specific probes.
利用针对麻疹病毒、犬瘟热病毒、风疹病毒和猿猴病毒5基因组RNA序列制备的单链35S标记RNA探针，对8例多发性硬化症（MS）患者以及56例非神经和神经疾病对照者的脑样本进行了原位杂交研究。在2例MS病例以及1例对照（1例播散性巨细胞病毒感染伴脊髓坏死）中，使用针对麻疹病毒核衣壳蛋白（N）、磷蛋白和融合蛋白基因序列的探针发现了杂交灶。使用针对麻疹病毒N基因组序列制备的生物素化探针证实了这一结果。在任何MS病例或对照病例中，使用其他任何病毒基因组特异性探针均未发现杂交现象。
