Department of Biochemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia.
School of Medicine, University of Split, Šoltanska 2, 21000 Split, Croatia.
Molecules. 2023 Jul 9;28(14):5299. doi: 10.3390/molecules28145299.
(Roth) G. Don., immortelle, is a plant species used in ethnomedicine and the food industry as a spice added to food, beverages, and bakery products. It has been shown to possess various biological activities, such as antioxidant and antibacterial activity, making it useful as a natural preservative. We investigated the phytochemical profile and biological activity of essential oils from wild-grown plant material collected from natural habitats in the Republic of Croatia and Bosnia and Herzegovina. Using high-resolution scanning electron microscopy (SEM), a visual investigation of plant organs (stem, leaf, and flower) was performed, confirming the presence of essential oil reservoirs on the surface of all examined plant organs. Essential oils were isolated by hydrodistillation in the Clevenger apparatus. The chemical composition of the essential oils was determined using the GC-MS analytical technique. Cytotoxic activity tests were performed in vitro on three cell lines: skin (fibroblast), lung, and breast cancer. Using statistical tools, the synergistic and selective effects of essential oil on healthy and tumor cells were correlated to chemical composition and cytotoxic activity. The synergistic and antagonistic effects of essential oil's individual components were simulated by testing pure compounds and their mixture of cytotoxic activity on fibroblasts and breast cancer cells. The results confirm that essential oil's biological activity is much greater than the sum of the effects of its components. The present data are novel contributions to the body of knowledge on the biological activity of this species used in the food industry.
(Roth) G. Don.，长生草，是一种在民族医学和食品工业中被用作香料的植物物种，添加到食品、饮料和烘焙产品中。已证明它具有多种生物活性，如抗氧化和抗菌活性，使其可用作天然防腐剂。我们研究了从克罗地亚和波斯尼亚和黑塞哥维那自然栖息地野生生长的植物材料中提取的精油的植物化学特征和生物活性。使用高分辨率扫描电子显微镜（SEM），对植物器官（茎、叶和花）进行了视觉研究，证实所有检查的植物器官表面都存在精油储库。精油通过 Clevenger 装置进行水蒸馏分离。使用 GC-MS 分析技术确定精油的化学成分。在体外对三种细胞系：皮肤（成纤维细胞）、肺和乳腺癌进行细胞毒性活性测试。使用统计工具，将精油对健康细胞和肿瘤细胞的协同和选择性作用与化学成分和细胞毒性活性相关联。通过测试纯化合物及其混合物对成纤维细胞和乳腺癌细胞的细胞毒性活性，模拟了精油的各个成分的协同和拮抗作用。结果证实，精油的生物活性远大于其成分的总和。本数据为该物种在食品工业中的生物活性的知识体系提供了新的贡献。
