Mastelić Josip, Politeo Olivera, Jerković Igor
Department of Organic Chemistry, Faculty of Chemistry and Technology, N. Tesle 10/V, 21000 Split, Croatia.
Molecules. 2008 Apr 7;13(4):795-803. doi: 10.3390/molecules13040795.
The essential oil of Helichrysum italicum (Roth) G. Don (everlasting or Immortelle essential oil) was isolated by hydrodistillation and analysed by GC and GCMS. Forty four compounds were identified. The main components were alpha-pinene(12.8%), 2-methyl-cyclohexyl pentanoate (11.1 %), neryl acetate (10.4%), 1,7-di-epi-alpha-cedrene (6.8%) and other compounds. The oil was fractionated and ester-containing fraction was hydrolysed with KOH/H(2)SO(4). The liberated volatiles were analysed by GC and GC-MS: three phenols and twenty seven volatile carboxylic acids were identified[70% low fatty acids (C(2)-C(5)), 15% C(10)-C(12) acids and 15% other acids]. The main acids were acetic acid (24.3%) propanoic acid (17.2%), 2-methylpropanoic acid (11.4%),dodecanoic acid (8.7%), 2-methylbutanoic acid (8.3%), (Z)-2-methylbutenoic acid(5.1%) and decanoic acid (4.6%). With respect to the identified bonded carboxylic acids,the minimal number of esters in the oil was twenty seven, but their overall quantity was probably larger due to different possible combinations of alcohols with acids to form esters. On the other hand, only six main esters were identified in the oil before fractionation and hydrolysis.
通过水蒸馏法分离出意大利蜡菊（Roth）G. Don的精油（永恒或不凋花精油），并采用气相色谱（GC）和气相色谱 - 质谱联用（GCMS）进行分析。共鉴定出44种化合物。主要成分是α-蒎烯（12.8%）、2 - 甲基环己基戊酸酯（11.1%）、乙酸橙花酯（10.4%）、1,7 - 二表-α-雪松烯（6.8%）以及其他化合物。对该精油进行分馏，含酯馏分用氢氧化钾/硫酸水解。对释放出的挥发性成分进行GC和GC - MS分析：鉴定出三种酚类和二十七种挥发性羧酸[70%为低级脂肪酸（C₂ - C₅），15%为C₁₀ - C₁₂酸，15%为其他酸]。主要的酸为乙酸（24.3%）、丙酸（17.2%）、2 - 甲基丙酸（11.4%）、十二烷酸（8.7%）、2 - 甲基丁酸（8.3%）、（Z）-2 - 甲基丁烯酸（5.1%）和癸酸（4.6%）。就鉴定出的结合羧酸而言，该精油中酯的最少数量为二十七个，但由于醇与酸形成酯的可能组合不同，其总量可能更大。另一方面，在分馏和水解之前，该精油中仅鉴定出六种主要酯类。
