School of Dentistry and Medical Sciences, Charles Sturt University, Bathurst, NSW 2795, Australia.
School of Science, Western Sydney University, Penrith, NSW 2751, Australia.
Molecules. 2023 Jul 14;28(14):5400. doi: 10.3390/molecules28145400.
This study investigated the effects of a modified rice bran arabinoxylan compound (RBAC) as a dietary supplement on the gut microbiota of healthy adults. Ten volunteers supplemented their diet with 1 g of RBAC for six weeks and 3 g of RBAC for another six weeks, with a three-week washout period. Faecal samples were collected every 3 weeks over 21 weeks. Microbiota from faecal samples were profiled using 16S rRNA sequencing. Assessment of alpha and beta microbiota diversity was performed using the QIIME2 platform. The results revealed that alpha and beta diversity were not associated with the experimental phase, interventional period, RBAC dosage, or time. However, the statistical significance of the participant was detected in alpha ( < 0.002) and beta (weighted unifrac, = 0.001) diversity. Explanatory factors, including diet and lifestyle, were significantly associated with alpha ( < 0.05) and beta ( < 0.01) diversity. The individual beta diversity of six participants significantly changed ( < 0.05) during the interventional period. Seven participants showed statistically significant taxonomic changes (ANCOM W ≥ 5). These results classified four participants as responders to RBAC supplementation, with a further two participants as likely responders. In conclusion, the gut microbiome is highly individualised and modulated by RBAC as a dietary supplement, dependent on lifestyle and dietary intake.
本研究旨在探讨改良米糠阿拉伯木聚糖复合物（RBAC）作为膳食补充剂对健康成年人肠道微生物群的影响。10 名志愿者在六周内补充 1 克 RBAC，然后再补充六周 3 克 RBAC，其间有三周的洗脱期。在 21 周的时间内，每 3 周采集一次粪便样本。使用 16S rRNA 测序对粪便样本中的微生物群进行分析。使用 QIIME2 平台评估 alpha 和 beta 多样性。结果表明，alpha 和 beta 多样性与实验阶段、干预期、RBAC 剂量或时间均无关联。然而，在 alpha 多样性（<0.002）和 beta 多样性（加权 unifrac，=0.001）中检测到了参与者的统计学意义。饮食和生活方式等解释因素与 alpha（<0.05）和 beta（<0.01）多样性显著相关。六名参与者的个体 beta 多样性在干预期内发生显著变化（<0.05）。七名参与者表现出统计学上显著的分类群变化（ANCOM W≥5）。这些结果将四名参与者归类为 RBAC 补充剂的应答者，另有两名参与者可能是应答者。综上所述，肠道微生物组具有高度个体化特征，并且受 RBAC 作为膳食补充剂的调节，这取决于生活方式和饮食摄入。
