Alessiani Alessandra, Di Domenico Marco, Averaimo Daniela, Pompilii Cinzia, Rulli Marco, Cocco Antonio, Lomellini Laura, Coccaro Antonio, Cantelmi Maria Chiara, Merola Carmine, Tieri Elga Ersilia, Romeo Gianfranco, Secondini Barbara, Marfoglia Cristina, Di Teodoro Giovanni, Petrini Antonio
Istituto Zooprofilattico Sperimentale di Abruzzo e Molise "G. Caporale", 64100 Teramo, Italy.
Department of Bioscience and Technology for Food and Environment, University of Teramo, 64100 Teramo, Italy.
Animals (Basel). 2024 Aug 2;14(15):2248. doi: 10.3390/ani14152248.
is the causative agent of Q fever. The main reservoirs for this bacterium, which can lead to human infection, in our region are typically cattle, goats, and sheep. In animals, infection is often detected due to reproductive problems. European Member States are required to report confirmed cases annually, but the lack of uniform reporting methods makes the data rather inconsistent. The Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise is involved in official controls to identify the causes of abortions, monitor suspected or positive herds, evaluate suspected infections in pets and humans, monitor the spread in wildlife, etc. In this paper, we summarize the presence of over the last five years (2019-2023). Additionally, a detailed overview of infection in wild and domestic animals is provided. Five hundred sixty animals-including cattle; goats; sheep; wild animals, such as deer, boars, wolves, roe deer, owls, and otters; buffalo; dogs; horses; cats; and a donkey-and six human samples were tested by real-time PCR on the transposase gene IS1111 to detect . The MST profile was identified in some of the samples. Outbreaks of occurred in four herds. In one of them, it was possible to follow the outbreak from inception to eradication by evaluating the effect of vaccination on real-time PCR Ct values. A total of 116 animals tested positive for , including 73 goats, 42 sheep, and one bovine. None of the other samples tested positive. The strains for which the ST was performed were identified as ST79, a strain that has been present in the area for more than ten years. The effect of vaccination on the reduction of positive samples and the variation of real-time PCR Ct values was evaluated in strict correlation.
是Q热的病原体。在我们地区，这种可导致人类感染的细菌的主要宿主通常是牛、山羊和绵羊。在动物中，感染通常因生殖问题而被检测到。欧洲成员国需要每年报告确诊病例，但报告方法缺乏统一使得数据相当不一致。阿布鲁佐和莫利塞实验动物卫生研究所参与官方控制，以确定流产原因、监测疑似或阳性畜群、评估宠物和人类的疑似感染、监测野生动物中的传播情况等。在本文中，我们总结了过去五年（2019 - 2023年）的存在情况。此外，还提供了野生动物和家畜感染的详细概述。通过对转座酶基因IS1111进行实时PCR检测，对560只动物（包括牛、山羊、绵羊、野生动物，如鹿、野猪、狼、狍、猫头鹰和水獭、水牛、狗、马、猫和一头驴）和6份人类样本进行检测以检测。在一些样本中鉴定出了MST图谱。在四个畜群中发生了疫情。在其中一个畜群中，通过评估疫苗接种对实时PCR Ct值的影响，有可能追踪疫情从开始到根除的过程。共有116只动物检测出阳性，其中包括73只山羊、42只绵羊和1头牛。其他样本均未检测出阳性。进行ST检测的菌株被鉴定为ST79，该菌株已在该地区存在十多年。严格关联评估了疫苗接种对减少阳性样本和实时PCR Ct值变化的影响。
