Family Genes from Positively Respond to Multiple Stresses.

The phytohormone abscisic acid (ABA) plays important roles in response to abiotic and biotic stresses in plants. Pyrabactin resistance 1-like (PYR/PYL) proteins are well-known as ABA receptors, which are responsible for ABA signal transduction. Nevertheless, the characteristics of genes from , an endangered timber tree, remain unclear in coping with various stresses. In this study, five s were identified from the genome of by sequence alignment and conserved motif analysis, which revealed that these s contain a conserved gate and latch motif for ABA binding. The LcPYL promoters possess a series of cis-acting elements involved in response to various hormone and abiotic stresses. Moreover, the transcriptome data of leaves reveal that genes specifically transcript under different abiotic stresses; transcription was induced by drought and cold treatment, and and transcription was upregulated under cold and hot stress, respectively. Meanwhile, the s with high expression levels shown in the transcriptomes were also found to be upregulated in whole plants treated with the same stresses tested by qPCR. Moreover, under biotic stress caused by scale insect and whitefly, leaves exhibited a distinct phenotype including disease spots that are dark green in the middle and yellow on the margin; the qPCR results showed that the relative expression levels of and in infected leaves were upregulated by 1.76 and 3.75 folds relative to normal leaves, respectively. The subcellular localizations of these stress-responsive LcPYLs were also identified in protoplasts of . These results provide a foundation to elucidate the function of s from this elite tree species and assist in understanding the molecular mechanism of in dealing with abiotic and biotic stresses. In future research, the detailed biological function of s and the genetic redundancy between s can be explored by gene overexpression and knockout based on this study.