Sun Xiao, Zhu Liming, Hao Zhaodong, Wu Weihuang, Xu Lin, Yang Yun, Zhang Jiaji, Lu Ye, Shi Jisen, Chen Jinhui
State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
Plants (Basel). 2023 May 30;12(11):2157. doi: 10.3390/plants12112157.
is a tree species of the family, an ancient relict plant mainly used for landscaping and timber production due to its excellent material properties and ornamental value. The cytokinin oxidase/dehydrogenase (CKX) enzyme regulates cytokinin levels and plays an important role in plant growth, development, and resistance. However, too-high or too-low temperatures or soil drought can limit the growth of , representing a key issue for research. Here, we identified the gene family in the genome and examined its transcriptional responses to cold, drought, and heat stresses. A total of five genes, distributed on four chromosomes and divided into three phylogenetic groups, were identified across the whole genome. Further analysis showed that multiple hormone- and stress-responsive cis-acting elements are located in the promoter regions of s, indicating a potential role of these s in plant growth, development, and response to environmental stresses. Based on existing transcriptome data, s, especially , were found to transcriptionally respond to cold, heat, and drought stresses. Furthermore, quantitative reverse-transcription PCR (qRT-PCR) showed that responds to drought stress in an ABA-dependent manner in stems and leaves and in an ABA-independent manner in roots. These results lay a foundation for functional research on genes in the resistance breeding of the rare and endangered tree species of .
是该科的一种树种，是一种古老的孑遗植物，因其优良的材质特性和观赏价值，主要用于园林绿化和木材生产。细胞分裂素氧化酶/脱氢酶（CKX）调节细胞分裂素水平，在植物生长、发育和抗性中起重要作用。然而，过高或过低的温度或土壤干旱会限制其生长，这是一个关键的研究问题。在这里，我们在其基因组中鉴定了该基因家族，并研究了其对冷、旱和热胁迫的转录响应。在整个基因组中总共鉴定出五个该基因，分布在四条染色体上并分为三个系统发育组。进一步分析表明，多个激素和胁迫响应顺式作用元件位于这些基因的启动子区域，表明这些基因在植物生长、发育和对环境胁迫的响应中具有潜在作用。基于现有的转录组数据，发现这些基因，尤其是该基因，在转录水平上对冷、热和干旱胁迫有响应。此外，定量逆转录PCR（qRT-PCR）表明，该基因在茎和叶中以依赖脱落酸的方式对干旱胁迫作出反应，而在根中以不依赖脱落酸的方式作出反应。这些结果为该珍稀濒危树种抗性育种中该基因的功能研究奠定了基础。
