Song Meiqi, Lu Ping, Yin Yue, Zhang Xuewei, Ge Wei, Zhang Kezhong
College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
Beijing Laboratory of Urban and Rural Ecological Environment, Beijing, 102206, China.
Planta. 2025 Aug 5;262(3):73. doi: 10.1007/s00425-025-04763-5.
Lilium species are widely used in horticulture for cut flowers and decorations as well as in traditional medicine for their ornamental, edible, and medicinal value. Although it is commonly known that variable temperature treatment could accelerate Lilium development from vegetative to reproductive growth, little is known about the molecular mechanisms of its development. Here, the key module miR396f-GRF10 was selected based on bioinformatics analysis, and transgenic function verification was conducted, which showed that overexpression of Lbr-miR396f inhibited the phase transition. In contrast, overexpression of LbrGRF10 promoted this process. In addition, through subcellular localization and bimolecular fluorescence complementation, the interaction of LbrGRF10 with LbrGIF1 and LbrGIF2 and its localization in the nucleus were confirmed. Results from in situ hybridization experiments revealed that after alternating temperature treatment, in the variable temperature treatment group, the hybridization signal exhibited higher expression levels in the apical growth cone, young leaves, and shoot axes, indicating a significant up-regulation of LbrGRF10 expression. This study provides new perspectives and ideas for revealing the molecular mechanisms of phase transition in Lilium.
百合属植物因其观赏、食用和药用价值，在园艺中被广泛用于切花和装饰，以及传统医学。虽然人们普遍知道变温处理可以加速百合从营养生长向生殖生长的发育，但对其发育的分子机制却知之甚少。在此，基于生物信息学分析选择了关键模块miR396f-GRF10，并进行了转基因功能验证，结果表明Lbr-miR396f的过表达抑制了阶段转变。相反，LbrGRF10的过表达促进了这一过程。此外，通过亚细胞定位和双分子荧光互补，证实了LbrGRF10与LbrGIF1和LbrGIF2的相互作用及其在细胞核中的定位。原位杂交实验结果显示，变温处理后，在变温处理组中，杂交信号在顶端生长锥、幼叶和茎轴中表现出较高的表达水平，表明LbrGRF10表达显著上调。本研究为揭示百合阶段转变的分子机制提供了新的视角和思路。
