Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan.
Glycoscience, Central Research Laboratory, Seikagaku Corporation, 3-1253, Tateno, Higashiyamato, Tokyo, 207-0021, Japan.
Sci Rep. 2023 Jul 29;13(1):12313. doi: 10.1038/s41598-023-38849-5.
Chondroitin sulfate (CS) is a family of glycosaminoglycans and have a wide range of applications in dietary supplements and pharmaceutical drugs. In this study, we evaluated the effects of several types of CS, differing in their sulfated positions, on the human colonic microbiota and their metabolites. CS (CSA, CSC, and CSE) and non-sulfated chondroitin (CH) were added into an in vitro human colonic microbiota model with fecal samples from 10 healthy individuals. CS addition showed a tendency to increase the relative abundance of Bacteroides, Eubacterium, and Faecalibacterium, and CSC and CSE addition significantly increased the total number of eubacteria in the culture of the Kobe University Human Intestinal Microbiota Model. CSE addition also resulted in a significant increase in short-chain fatty acid (SCFA) levels. Furthermore, addition with CSC and CSE increased the levels of a wide range of metabolites including lysine, ornithine, and Ile-Pro-Pro, which could have beneficial effects on the host. However, significant increases in the total number of eubacteria, relative abundance of Bacteroides, and SCFA levels were also observed after addition with CH, and the trends in the effects of CH addition on metabolite concentrations were identical to those of CSC and CSE addition. These results provide novel insight into the contribution of the colonic microbiota to the beneficial effects of dietary CS.
硫酸软骨素（CS）是一类糖胺聚糖，在膳食补充剂和药物中有广泛的应用。在这项研究中，我们评估了几种 CS，它们在硫酸化位置上有所不同，对人类结肠微生物群及其代谢物的影响。CS（CSA、CSC 和 CSE）和非硫酸化软骨素（CH）被添加到一个体外人类结肠微生物群模型中，该模型使用来自 10 个健康个体的粪便样本。CS 的添加趋势增加了拟杆菌、真杆菌和粪杆菌的相对丰度，CSC 和 CSE 的添加显著增加了 Kobe 大学人类肠道微生物群模型培养物中总真细菌的数量。CSE 的添加还导致短链脂肪酸（SCFA）水平显著增加。此外，CSC 和 CSE 的添加增加了包括赖氨酸、鸟氨酸和 Ile-Pro-Pro 在内的多种代谢物的水平，这些代谢物可能对宿主有益。然而，在添加 CH 后，总真细菌数量、拟杆菌相对丰度和 SCFA 水平也显著增加，并且 CH 添加对代谢物浓度的影响趋势与 CSC 和 CSE 添加相同。这些结果为膳食 CS 的有益作用是由结肠微生物群贡献提供了新的见解。
