Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, PR China.
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, PR China.
Int J Biol Macromol. 2019 Jan;121:981-988. doi: 10.1016/j.ijbiomac.2018.10.100. Epub 2018 Oct 18.
In the present study, the physicochemical properties of polysaccharides from corn silk (CSP) with different molecular weights were characterized and their inhibitory actions against α-glucosidase and α-amylase were evaluated. Then their effects on glucose uptake and mechanism of action in PI3K/Akt signaling pathway were also determined in L6 skeletal muscle cells. Results demonstrated that CSP could significantly inhibit α-amylase and α-glucosidase. CSP were non-toxic and they considerably increased the 2-NBDG uptake (P < 0.05) in L6 cells. Additionally, CSP regulated the PI3K/Akt signaling pathway and promoted the translocation of GLUT4 to the plasma membrane that would enhanced the glucose uptake in rat L6 skeletal muscle cells. Among the tested polysaccharides, CSP2 with the molecular weight of 62.16 kDa exerted appreciable hypoglycemic activity. Altogether it was suggested that CSP could be the promising candidate in the treatment of Type 2 Diabetes Mellitus (T2DM). The elucidation of the potential anti-diabetic mechanism of the polysaccharides from corn silk will facilitate their utilization in the functional food industries.
在本研究中，对具有不同分子量的玉米须多糖（CSP）的理化性质进行了表征，并评价了其对α-葡萄糖苷酶和α-淀粉酶的抑制作用。然后，在 L6 骨骼肌细胞中测定了它们对葡萄糖摄取的影响及其在 PI3K/Akt 信号通路中的作用机制。结果表明，CSP 能显著抑制α-淀粉酶和α-葡萄糖苷酶。CSP 无毒性，可显著增加 L6 细胞中 2-NBDG 的摄取（P<0.05）。此外，CSP 调节 PI3K/Akt 信号通路，促进 GLUT4 向质膜易位，从而增强大鼠 L6 骨骼肌细胞的葡萄糖摄取。在所测试的多糖中，分子量为 62.16 kDa 的 CSP2 表现出明显的降血糖活性。总之，CSP 可能是治疗 2 型糖尿病（T2DM）的有前途的候选药物。阐明玉米须多糖的潜在抗糖尿病机制将有助于其在功能性食品工业中的应用。
