Department of Preventive Medicine, Konyang University College of Medicine, Daejeon, South Korea.
Department of Immunology, Jeonbuk National University Medical School, Jeonju, South Korea.
Am J Trop Med Hyg. 2023 Jul 31;109(3):554-558. doi: 10.4269/ajtmh.23-0269. Print 2023 Sep 6.
Virus-induced severe fever with thrombocytopenia syndrome (SFTS) induces a cell-mediated immune response that likely contributes to virus control in SFTS patients. To identify the temporal changes of the cell-mediated immune response, we investigated the changes in serum levels of perforin and granzymes at early periods after illness onset in SFTS patients. We analyzed 32 SFTS patients and compared the temporal patterns of serum perforin and granzyme A and B to that of 20 healthy control adults using the Mann-Whitney U test. Compared with healthy controls, the mean level of perforin was significantly reduced by 81% (P < 0.01) during the first week after illness onset, whereas granzyme B significantly increased by 4.6-fold (P = 0.02) in the first week after illness onset and decreased to normal afterward. During the study period, there was no significant difference in serum perforin and granzyme. These findings indicate that perforin and granzyme B in serum can be considered possible serologic markers that reflect the clinical stage of SFTS. Additional study is warranted for tracking circulating perforin and granzyme in different ages and for an extended period after illness onset.
病毒诱导的发热伴血小板减少综合征（SFTS）会引起细胞介导的免疫反应，这可能有助于 SFTS 患者控制病毒。为了确定细胞介导的免疫反应的时间变化，我们研究了 SFTS 患者发病早期血清中穿孔素和颗粒酶水平的变化。我们分析了 32 名 SFTS 患者，并使用曼-惠特尼 U 检验将血清穿孔素和颗粒酶 A 和 B 的时间模式与 20 名健康对照成年人进行了比较。与健康对照组相比，发病后第一周穿孔素水平显著降低 81%（P<0.01），而颗粒酶 B 在发病后第一周显著增加 4.6 倍（P=0.02），随后降至正常水平。在研究期间，血清穿孔素和颗粒酶没有显著差异。这些发现表明，血清中的穿孔素和颗粒酶 B 可以作为反映 SFTS 临床阶段的可能的血清学标志物。需要进一步研究以跟踪不同年龄和发病后延长时间的循环穿孔素和颗粒酶。
