Department of Clinical Chemistry, Graduate School of Health and Social Services, Saitama Prefectural University, Koshigaya, Japan.
Department of Clinical Laboratory, The University of Tokyo Hospital, Bunkyo-ku, Japan.
Ann Clin Biochem. 2024 Mar;61(2):90-97. doi: 10.1177/00045632231194829. Epub 2023 Aug 30.
Blood collection tubes with sodium fluoride (NaF) added as a glycolytic inhibitor are widely used for glucose measurement. However, the glycolytic inhibitory effects of NaF are insufficient, and decreases in glucose levels over time after blood collection have become a problem.
Blood from a volunteer collected using an NaF tube was used to compare the glycolysis inhibitory abilities of ATP and ADP. Blood samples from 10 volunteers were collected in NaF tubes and NaF tubes with added ATP (NaF-ATP tubes). The stability of glucose and haemoglobin (Hb)A1c after whole-blood storage from immediately after blood collection to 24 h later was compared.
ATP and ADP had similar inhibitory effects on glycolysis, but ATP was selected as an additive for blood collection tubes because ADP was more haemolytic than ATP. We verified the ability of NaF blood collection tubes supplemented with ATP to inhibit glycolysis. Mean (± standard deviation) glucose levels (n=10) after storage for 24 h after blood collection decreased to -9.0 ± 2.7 mg/dL (-0.50 ± 0.15 mmol/L) in conventional NaF tubes. NaF-ATP(20) tubes with 20 mg (0.036 mmol) ATP added showed a reduced decrease, with a mean of -5.8 ± 2.9 mg/dL (-0.32 ± 0.16 mmol/L). NaF-ATP tubes also had no effect on HbA1c measurement.
This study reports on a blood collection tube that enables the measurement of glucose and HbA1c. Based on the results of validation, we conclude that NaF-ATP tubes can reduce decreases in glucose over time in stored whole blood compared to conventional NaF tubes.
添加氟化钠 (NaF) 作为糖酵解抑制剂的血液采集管广泛用于葡萄糖测量。然而，NaF 的糖酵解抑制作用不足，并且采集后血液中葡萄糖水平随时间下降已成为一个问题。
使用含 NaF 管采集的志愿者血液比较 ATP 和 ADP 的糖酵解抑制能力。10 名志愿者的血液样本分别采集于含 NaF 的管（NaF 管）和添加 ATP 的 NaF 管（NaF-ATP 管）中。比较立即采集血液后至 24 小时后全血储存期间葡萄糖和血红蛋白 (HbA1c) 的稳定性。
ATP 和 ADP 对糖酵解具有相似的抑制作用，但由于 ADP 的溶血作用强于 ATP，因此选择 ATP 作为血液采集管的添加剂。我们验证了添加 ATP 的 NaF 血液采集管抑制糖酵解的能力。采集后 24 小时储存后（n=10），常规 NaF 管中的葡萄糖水平（平均值±标准差）下降至-9.0±2.7 mg/dL（-0.50±0.15 mmol/L）。添加 20mg（0.036mmol）ATP 的 NaF-ATP(20)管显示出减少的下降，平均值为-5.8±2.9 mg/dL（-0.32±0.16 mmol/L）。NaF-ATP 管对 HbA1c 测量也没有影响。
本研究报告了一种能够测量葡萄糖和 HbA1c 的血液采集管。基于验证结果，我们得出结论，与常规 NaF 管相比，NaF-ATP 管可以减少储存全血中葡萄糖随时间的下降。
