Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, China.
Zhuzhou Hetang District Center for Disease Control and Prevention, Zhuzhou, Hunan, China.
Anal Methods. 2023 Aug 24;15(33):4059-4065. doi: 10.1039/d3ay00950e.
A simple dual-signal assay that combined colorimetric and fluorometric strategy for uric acid (UA) rapid detection was designed based on the versatility of facile synthesized MnO nanosheet. The oxidization of 3,3',5,5'-tetramethylbenzidine (TMB) and the fluorescence quenching of quantum dots (QDs) occurred simultaneously in the presence of MnO nanosheet. UA could decompose MnO nanosheet into Mn, resulting in the fluorescence recovery of QDs, along with the fading of the blue color of ox TMB. Based on the principles above, the detection of UA could be realized by the change of the dual signals (colorimetric and fluorometric). The linear range of the colorimetric mode was 5-60 μmol L, and the limit of detection (LOD) was 2.65 μmol L; the linear range of the fluorescence mode was wide at 5-120 μmol L, and the LOD could be as low as 1.33 μmol L. The method was successfully used for analyzing UA levels in human serum samples, indicating that this new dual-signal method could be applied in clinical diagnosis.
基于 MnO 纳米片的多功能性，设计了一种简单的双重信号测定法，将比色和荧光策略相结合，用于快速检测尿酸 (UA)。在 MnO 纳米片的存在下，3,3',5,5'-四甲基联苯胺 (TMB) 的氧化和量子点 (QDs) 的荧光猝灭同时发生。UA 可将 MnO 纳米片分解成 Mn，导致 QDs 的荧光恢复，同时 oxTMB 的蓝色褪色。基于上述原理，通过双重信号（比色和荧光）的变化可以实现 UA 的检测。比色模式的线性范围为 5-60 μmol L，检测限 (LOD) 为 2.65 μmol L；荧光模式的线性范围较宽，为 5-120 μmol L，LOD 可低至 1.33 μmol L。该方法成功用于分析人血清样品中的 UA 水平，表明这种新的双重信号方法可应用于临床诊断。
