MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, China.
Laboratory of Gynecologic Oncology, Department of Gynecology, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, China.
Mikrochim Acta. 2023 May 13;190(6):217. doi: 10.1007/s00604-023-05767-2.
Serum levels of uric acid (UA) play an important role in the prevention of diseases. Developing a rapid and accurate way to detect UA is still a meaningful task. Hence, positively charged manganese dioxide nanosheets (MnONSs) with an average latter size of 100 nm and an ultra-thin thickness of below 1 nm have been prepared. They can be well dispersed in water and form stable yellow-brown solutions. The MnONSs can be decomposed by UA via redox reaction, leading to a decline of a characteristic absorption peak (374 nm) and a color fading of MnONSs solution. On this basis, an enzyme-free colorimetric sensing system for the detection of UA has been developed. The sensing system shows many advantages, including a wide linear range of 0.10-50.0 μmol/L, a limit of quantitation (LOQ) of 0.10 μmol/L, a low limit of detection (LOD) of 0.047 μmol/L (3σ/m), and rapid response without need of strict time control. Moreover, a simple and convenient visual sensor for UA detection has also been developed by adding an appropriate amount of phthalocyanine to provide a blue background color, which helps to increase visual discrimination. Finally, the strategy has been successfully applied to detect UA in human serum and urine samples.
血清尿酸（UA）水平在疾病预防中起着重要作用。开发一种快速准确的 UA 检测方法仍然是一项有意义的任务。因此，制备了平均粒径为 100nm、厚度在 1nm 以下的带正电荷的二氧化锰纳米片（MnONSs）。它们可以很好地分散在水中，并形成稳定的黄棕色溶液。MnONSs 可以通过氧化还原反应被 UA 分解，导致特征吸收峰（374nm）下降和 MnONSs 溶液褪色。在此基础上，建立了一种无需酶的用于检测 UA 的比色传感体系。该传感体系具有许多优点，包括 0.10-50.0 μmol/L 的宽线性范围、0.10 μmol/L 的定量下限（LOQ）、0.047 μmol/L（3σ/m）的低检测限（LOD）和快速响应，无需严格的时间控制。此外，通过向体系中添加适量的酞菁，提供了一个蓝色的背景颜色，有助于提高视觉辨别度，还开发了一种用于 UA 检测的简单便捷的可视化传感器。最后，该策略已成功应用于检测人血清和尿液样本中的 UA。
