Abdoulahi Mahamane Idi Issa, Yanick Kevin Melogmo Dongmo, Lauve Rachel Tchokouaha Yamthe, Habibou Hama Hamadou, Sahabi Bakasso, Abdelkader Alio Sanda, Boyom Fabrice Fekam, Tidjani Ilagouma Amadou
Laboratory of Natural Substances and Organic Synthesis, FAST, Abdou Moumouni University, BP 10662, Niamey, Niger.
Laboratory for Phytobiochemistry and Medicinal Plants Studies, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
J Trop Med. 2023 Jul 24;2023:6120255. doi: 10.1155/2023/6120255. eCollection 2023.
The emergence of multidrug bacterial resistance poses a great public health problem and requires a constant search for new antibacterial agents. However, Niger's flora possesses several medicinal plants used in traditional medicine to cure infectious diseases and can be used as sources of bioactive ingredients. This current study was designed to evaluate the antibacterial activity of eight plants used in the traditional pharmacopeia of Niger. The extracts were prepared by maceration using ethanol, methanol, and distilled water. The obtained extracts were screened against spp., spp., and using the microdilution method coupled with a resazurin-based assay. Phytochemical screening was performed using colorimetry, while the quantification of total polyphenols, total flavonoids, and total tannins was determined by spectrophotometry. Out of the eight plants obtained, five named , , , , and exhibited antibacterial activity with MICs ranging from 500 g/mL to 2000 g/mL. Phytochemical screening showed the presence of alkaloids, saponosides, tannins, flavonoids, terpenes/sterols, quinones, and polyphenols. The ethanolic and methanolic extracts of contained important quantities of total polyphenols, with 43.59 ± 0.15 and 41.97 ± 0.02 mg EAG/100 mg of extract, respectively. These extracts showed the highest contents of total tannins at 46.49 g/L and 45.52 g/L, respectively. For total flavonoids, the highest content was obtained with the methanolic extract of , with 3.12 ± 0.01 mg QE/100 mg of extract. These findings justify the uses of these plants in traditional medicine for the treatment of infectious diseases such as diarrhea and can be used as starting points for the development of phytodrugs against infectious diarrhea.
多重耐药细菌的出现构成了一个重大的公共卫生问题，需要不断寻找新的抗菌剂。然而，尼日尔的植物群拥有几种用于传统医学治疗传染病的药用植物，可作为生物活性成分的来源。本研究旨在评估尼日尔传统药典中使用的八种植物的抗菌活性。通过用乙醇、甲醇和蒸馏水浸渍来制备提取物。使用微量稀释法结合基于刃天青的测定法，针对金黄色葡萄球菌、大肠杆菌和白色念珠菌对获得的提取物进行筛选。使用比色法进行植物化学筛选，而总多酚、总黄酮和总单宁的定量通过分光光度法测定。在所获得的八种植物中，五种名为A. indica、C. odorata、L. inermis、O. basilicum和T. occidentalis表现出抗菌活性，最低抑菌浓度范围为500μg/mL至2000μg/mL。植物化学筛选表明存在生物碱、皂苷、单宁、黄酮类化合物、萜类/甾醇、醌类和多酚类。印度楝的乙醇提取物和甲醇提取物含有大量的总多酚，分别为43.59±0.15和41.97±0.02mg EAG/100mg提取物。这些提取物的总单宁含量最高，分别为46.49g/L和45.52g/L。对于总黄酮，最高含量是用罗勒的甲醇提取物获得的，为3.12±0.01mg QE/100mg提取物。这些发现证明了这些植物在传统医学中用于治疗腹泻等传染病的用途，并且可以作为开发抗传染性腹泻植物药的起点。
