State University of Londrina, Department of Biochemistry and Biotechnology, P.O. Box 10, 011, 86057-970, Londrina, Paraná, Brazil.
State University of Londrina, Department of Pathological Sciences, P.O. Box 10, 011, 86057-970, Londrina, Paraná, Brazil.
Braz J Microbiol. 2023 Sep;54(3):2219-2226. doi: 10.1007/s42770-023-01081-4. Epub 2023 Aug 2.
Corn contamination with Fusarium verticillioides (Sacc.) Nirenberg is a worldwide problem that affects yield and grain quality resulting in severe economic losses and implications for food safety. Control of F. verticillioides is a challenge, but lactic acid bacteria (LAB) has high potential as a biological control agent. In this study, the antifungal effect of Limosilactobacillus reuteri (formerly Lactobacillus reuteri) LR-92 against F. verticillioides 97L was investigated. Cell-free supernatant (CFS) from L. reuteri showed concentration-dependent fungicidal and fungistatic activity against F. verticillioides 97L. The antifungal compounds from CFS showed heat stability and pH dependence, and antifungal activity was not affected by treatment with proteolytic enzymes. High-performance liquid chromatography analysis indicated that L. reuteri LR-92 produces lactic and acetic acids. After liquid-liquid extraction, electrospray ionization mass spectrometry analysis of the active ethyl acetate fraction containing antifungal compounds revealed the production of 3-phenyllactic acid, cyclo-(L-Pro-L-Leu), cyclo-(L-Pro-L-Phe), and cyclo-(L-Phe-trans-4-OH-L-Pro). L. reuteri LR-92 has potential as a biocontrol agent for F. verticillioides and contributes to food safety.
玉米感染串珠镰刀菌（Sacc.）是一个全球性的问题，会影响产量和谷物质量，导致严重的经济损失，并对食品安全产生影响。镰刀菌的防治是一个挑战，但乳酸菌（LAB）作为一种生物防治剂具有很大的潜力。在这项研究中，研究了鼠李糖乳杆菌（以前称为雷特氏乳杆菌）LR-92 对 97L 串珠镰刀菌的抗真菌作用。鼠李糖乳杆菌的无细胞上清液（CFS）对 97L 串珠镰刀菌表现出浓度依赖性的杀菌和抑菌活性。CFS 中的抗真菌化合物具有热稳定性和 pH 依赖性，并且抗真菌活性不受蛋白酶处理的影响。高效液相色谱分析表明，鼠李糖乳杆菌 LR-92 产生乳酸和乙酸。经过液液萃取后，对含有抗真菌化合物的活性乙酸乙酯馏分进行电喷雾电离质谱分析，揭示了 3-苯乳酸、环（L-Pro-L-Leu）、环（L-Pro-L-Phe）和环（L-Phe-trans-4-OH-L-Pro）的产生。鼠李糖乳杆菌 LR-92 具有作为串珠镰刀菌生物防治剂的潜力，有助于食品安全。
