Jørgensen Mette Rose, Kragelund Camilla, Jensen Peter Østrup, Keller Mette Kirstine, Twetman Svante
Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen , Copenhagen , Denmark.
Department of Clinical Microbiology, Rigshospitalet , Copenhagen , Denmark.
J Oral Microbiol. 2017 Jan 18;9(1):1274582. doi: 10.1080/20002297.2016.1274582. eCollection 2017.
An alternative approach for managing infections in the oral cavity by modulating the oral microbiota with probiotic bacteria has been proposed. The aim was to investigate the antifungal potential of the probiotic bacterium (DSM 17938 and ATCC PTA 5289) against six oral species (, and ). The lactobacilli were tested for their ability to co-aggregate with and inhibit the growth of the yeasts assessed by spectrophotometry and the agar overlay inhibition assay. Additionally, the pH was evaluated with microsensors, and the production of hydrogen peroxide (H2O2) by the lactobacilli was verified. Both strains showed co-aggregation abilities with the yeasts. The lactobacilli almost completely inhibited the growth of and , but did not affect . Statistically significant differences in co-aggregation and growth inhibition capacities between the two strains were observed (p<0.001). The pH measurements suggested that can resist the acids produced by the lactobacilli. exhibited antifungal properties against five of the six most common oral Candida species. Further, the results reconfirms that the probiotic capacity of is strain specific.
有人提出了一种通过用益生菌调节口腔微生物群来管理口腔感染的替代方法。目的是研究益生菌（DSM 17938和ATCC PTA 5289）对六种口腔菌种（、和）的抗真菌潜力。通过分光光度法和琼脂覆盖抑制试验评估了乳酸杆菌与酵母共聚集以及抑制酵母生长的能力。此外，用微传感器评估了pH值，并验证了乳酸杆菌产生过氧化氢（H2O2）的情况。两种菌株均显示出与酵母的共聚集能力。乳酸杆菌几乎完全抑制了和的生长，但对没有影响。观察到两种菌株在共聚集和生长抑制能力方面存在统计学显著差异（p<0.001）。pH测量表明可以抵抗乳酸杆菌产生的酸。对六种最常见口腔念珠菌中的五种表现出抗真菌特性。此外，结果再次证实的益生菌能力具有菌株特异性。
