不同年龄供体匹配脂肪来源干细胞的抗衰老机制。
Anti-aging mechanism of different age donor-matched adipose-derived stem cells.
机构信息
Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology, Research Center for Biomedical Sciences, School of Life Sciences, Guangxi Normal University, Guilin, 541004, China.
Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland.
出版信息
Stem Cell Res Ther. 2023 Aug 2;14(1):192. doi: 10.1186/s13287-023-03415-3.
BACKGROUND
Adipose-derived stem cells (ASCs) have anti-aging and anti-obesity effects in aged animals, but the underlying molecular mechanism remains unknown.
METHODS
In the present study, we evaluated the in vivo transplantation effects of different age donor-matched ASCs on natural aging and leptin knockout mice (ob/ob mice). The multi-omics expression profiles of young and aged mouse donor-derived ASCs were also analyzed.
RESULTS
The results revealed that ASCs from young donors induced weight and abdominal fat loss for older recipients but not for young or ob/obmice. The young and aged mouse donor ASCs displayed significant phenotypic differences, contributing to the distinguished weight loss and anti-aging effects in aged mice.
CONCLUSIONS
Our data suggest an underlying molecular mechanism by which young-donor ASCs reduce immune cells and inflammation in aged mice via secreted immune factors. These findings point to a general anti-aging mechanism of stem cells, which may provide new insights into age-related disturbances of stem cell plasticity in healthy aging and age-related diseases.
背景
脂肪来源的干细胞（ASCs）在老年动物中具有抗衰老和抗肥胖作用，但潜在的分子机制尚不清楚。
方法
在本研究中，我们评估了不同年龄供体匹配的 ASC 对自然衰老和瘦素敲除小鼠（ob/ob 小鼠）的体内移植效果。还分析了年轻和年老的供体来源 ASC 的多组学表达谱。
结果
结果表明，来自年轻供体的 ASC 诱导老年受体体重和腹部脂肪减少，但对年轻或 ob/ob 小鼠没有作用。年轻和年老的供体来源的 ASC 表现出显著的表型差异，导致老年小鼠体重减轻和抗衰老效果的明显差异。
结论
我们的数据表明，年轻供体的 ASC 通过分泌的免疫因子减少老年小鼠中的免疫细胞和炎症，从而发挥作用。这些发现为干细胞的一般抗衰老机制提供了依据，这可能为健康衰老和与年龄相关的疾病中与年龄相关的干细胞可塑性紊乱提供新的见解。
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