Department of Genetics and Development, Institute for Cancer Genetics, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA.
Cell Rep Methods. 2023 Jul 24;3(7):100541. doi: 10.1016/j.crmeth.2023.100541.
In a recent issue of , Martin-Rufino et al. develop a strategy for performing high-throughput base-editing CRISPR screens coupled with single-cell readouts in the context of human hematopoiesis. Through a series of proof-of-principle experiments, the authors demonstrate the potential of base-editing screens for the study and treatment of hematological disorders.
在最近一期的《》杂志中，Martin-Rufino 等人提出了一种在人类造血过程中进行高通量碱基编辑 CRISPR 筛选并结合单细胞读数的策略。通过一系列原理验证实验，作者展示了碱基编辑筛选在血液病研究和治疗中的潜力。
