University of the Sunshine Coast (UniSC), 90 Sippy Downs Dr, Sippy Downs, QLD 4556, Australia.
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2023 Aug;73(8). doi: 10.1099/ijsem.0.005952.
Strains USC-21046 and USC-21048 were isolated from foaming coastal marine waters on the Sunshine Coast, Queensland, Australia. Both strains displayed growth and morphological characteristics typical for members belonging to the genus . The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine, and the major fatty acids were C, C ω9, C and C 10-methyl. The mycolic acids of strains USC-21046 and USC-21048 consisted of chain lengths between 50-64 and 56-68, respectively. Moreover, both of those strains contained -diaminopimelic acid and ribose, arabinose, glucose and galactose as whole cell sugars. Based on the phylogenomic results, both strains belonged to the genus with strain USC-21046 showing an 80.4 % genome similarity to NBRC 16497 and NBRC 100343, whereas USC-21048 strain showed an 83.6 % genome similarity to NBRC 100429. Both strains were delineated from their closely related relatives based on physiological (e.g. growth on sole carbon source) and chemotaxonomic (e.g. cellular fatty composition) differences. The digital DNA-DNA hybridization (dDDH) values between USC-21046 and USC-21048 and their closely related relatives were below the dDDH threshold value of ≤70 % used for the taxonomic classification of novel species status. The genome length of strains USC-21046 and USC-21048 were 6 878 863 and 7 066 978 bp, with G+C contents of 65.2 and 67.8 mol%, respectively. For the novel isolates, we propose the names sp. nov. with the type strain USC-21046 (=DSM 111727=NCCB 100867) and sp. nov. with the type strain USC-21048 (=DSM 111726=NCCB 100868).
菌株 USC-21046 和 USC-21048 从澳大利亚昆士兰州阳光海岸的泡沫沿海海域中分离出来。这两种菌株的生长和形态特征均表现出属于 属的特征。主要的极性脂是双磷脂酰甘油和磷脂酰乙醇胺，主要脂肪酸是 C、C ω9、C 和 C 10-甲基。菌株 USC-21046 和 USC-21048 的分枝菌酸链长分别为 50-64 和 56-68。此外，这两种菌株均含有 -二氨基庚二酸和核糖、阿拉伯糖、葡萄糖和半乳糖作为全细胞糖。基于基因组系统发育分析，两种菌株均属于 属，菌株 USC-21046 与 NBRC 16497 和 NBRC 100343 的基因组相似度为 80.4%，而 USC-21048 菌株与 NBRC 100429 的基因组相似度为 83.6%。基于生理（例如，唯一碳源上的生长）和化学分类学（例如，细胞脂肪酸组成）差异，这两种菌株与亲缘关系密切的相关菌株相区分。菌株 USC-21046 和 USC-21048 与其亲缘关系密切的相关菌株之间的数字 DNA-DNA 杂交（dDDH）值低于用于新型种状态分类的 dDDH 阈值（≤70%）。菌株 USC-21046 和 USC-21048 的基因组长度分别为 6878863bp 和 7066978bp，G+C 含量分别为 65.2%和 67.8%。对于新型分离株，我们提议使用模式菌株 USC-21046（=DSM 111727=NCCB 100867）和 USC-21048（=DSM 111726=NCCB 100868）分别命名为 sp. nov.。
