State Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing, China.
Renhe Global (Shanghai) Big Health Research Institute Co., LTD., Shanghai, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2023 Oct;272:109712. doi: 10.1016/j.cbpc.2023.109712. Epub 2023 Aug 5.
Probiotic Bacillus subtilis has beneficial efficacy on host's health. The microbiota-gut-blood system (MGBS) plays a crucial role in maintaining the homeostasis of hosts. However, the mechanism by which the probiotic B. subtilis positively acts on the MGBS of hosts remains unclear. Herein, we used an interspecies animal model to explore the causal associations between this bacterium and the micro-ecology balance and circulatory homeostasis of hosts. Results showed that the body weight of hosts significantly increased after probiotic B. subtilis supplementation (P < 0.05). Enterococcus was found to be the most important microbial marker causing the intergroup differences observed herein, and its relative abundance remarkably increased after B. subtilis supplementation. In addition, the supplementation of B. subtilis induced significant alterations in the levels of circulating metabolites, such as serine, arginine, adenine, uric acid, and pyridoxal (P < 0.05), indicating that B. subtilis modulated the metabolic profile of blood circulation in the host. The metabolisms of amino acids, purine, and vitamin B were the primary pathways modulated by B. subtilis. In conclusion, probiotic B. subtilis substantially introduced subtle but positive changes in the host's gut microbiome, and it promoted the physiological activity of the host by modulating circulating metabolites. The study provides a theoretical reference for the application of probiotic B. subtilis to improve the health state of specific populations.
枯草芽孢杆菌对宿主健康具有有益功效。微生物群-肠道-血液系统（MGBS）在维持宿主内环境平衡方面起着至关重要的作用。然而，益生菌枯草芽孢杆菌对宿主 MGBS 产生积极作用的机制尚不清楚。在此，我们使用种间动物模型来探索该细菌与宿主微生态平衡和循环内稳态之间的因果关系。结果表明，益生菌枯草芽孢杆菌补充后宿主体重显著增加（P<0.05）。发现肠球菌是导致本文观察到的组间差异的最重要微生物标志物，其相对丰度在枯草芽孢杆菌补充后显著增加。此外，枯草芽孢杆菌的补充诱导了循环代谢物水平的显著变化，如丝氨酸、精氨酸、腺嘌呤、尿酸和吡哆醛（P<0.05），表明枯草芽孢杆菌调节了宿主血液循环的代谢特征。枯草芽孢杆菌调节的主要代谢途径为氨基酸、嘌呤和维生素 B 的代谢。总之，益生菌枯草芽孢杆菌使宿主肠道微生物组发生了微妙但积极的变化，并通过调节循环代谢物来促进宿主的生理活性。该研究为益生菌枯草芽孢杆菌应用于改善特定人群的健康状态提供了理论参考。
