Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, 625021, India.
Department of Botany, Bishop Heber College (Autonomous), Affi. To Bharathidasan University, Trichy, Tamil Nadu, 620017, India.
Curr Microbiol. 2023 Aug 7;80(9):314. doi: 10.1007/s00284-023-03343-8.
Salmonella enterica is one of the foodborne pathogens that can infect humans, spreading from one person to another by contaminated food and water. To identify the pathogenic S. enterica from the contaminated food product, culture-based and molecular identifications, drug resistance profiling, virulence and genetic traits of the strains have been used. Herein, different animal products was subjected to screen for S. enterica prevalence, pathogenic characterization and compared with clinical Salmonella isolates (human). A total of 173 isolates from animal products and 51 isolates from clinical samples were collected. S. Typhi, S. Agona and S. Ohio were predominant serovars in blood, stool and different animal products. Both, clinical [37% (n = 19/51)] and animal product-associated isolates [21% (n = 37/173)] expressed their highest resistance to nalidixic acid. Thirty-one percentage of (n = 16/51) clinical isolates and 12% (n = 21/173) animal food-associated isolates were resistant to multiple classes of antibiotics. Class 1 integrons encoded by S. Typhi, S. Infantis and S. Emek were screened for sequence analysis, the result revealed that the cassettes encoded-aminoglycoside acetyltransferase and dihydrofolate reductase enzymes. Salmonella pathogenicity island-1 encoded-hilA gene was detected most frequently in all the isolates. PFGE profile revealed the genetic traits of the isolates which were closely linked with antibiotic-resistant properties and virulent characteristics. Only S. Enteritidis, collected from different samples had clonal similarities. In summary, drug-resistant pathogenic Salmonella prevalence was observed in the animal product that could be an important alarm to consumers with the risk of enteric fever and it causes the potential risk to public health.
肠炎沙门氏菌是一种食源性病原体，可以感染人类，通过受污染的食物和水在人与人之间传播。为了从污染的食品中鉴定出致病性肠炎沙门氏菌，已经使用了基于培养的和分子鉴定、药物耐药性分析、菌株的毒力和遗传特征。在此，对不同的动物产品进行了肠炎沙门氏菌流行率的筛选、致病性特征分析，并与临床沙门氏菌分离株（人类）进行了比较。共从动物产品中收集了 173 株分离株，从临床样本中收集了 51 株分离株。在血液、粪便和不同动物产品中，主要血清型为伤寒沙门氏菌、肠炎沙门氏菌和俄亥俄沙门氏菌。临床[37%（n=19/51）]和动物产品相关分离株[21%（n=37/173）]均对萘啶酸表现出最高的耐药性。31%（n=16/51）的临床分离株和 12%（n=21/173）的动物食品相关分离株对多类抗生素耐药。对伤寒沙门氏菌、婴儿沙门氏菌和埃马克沙门氏菌进行了携带的类 1 整合子的序列分析，结果表明，这些整合子编码了氨基糖苷乙酰转移酶和二氢叶酸还原酶。所有分离株均检测到沙门氏菌致病岛-1 编码的 hilA 基因。PFGE 图谱显示了分离株的遗传特征，这些特征与抗生素耐药性和毒力特征密切相关。只有从不同样本中采集的肠炎沙门氏菌具有克隆相似性。总之，在动物产品中观察到了耐药性致病性沙门氏菌的流行，这可能对消费者构成肠热病的重要警报，并对公共健康构成潜在威胁。
