Piechota Dorota, Wardaszka Patrycja, Barańska-Rybak Wioletta, Jaœkiewicz Maciej, Kamysz Wojciech, Deptuła Milena, Pikuła Michał, Ostaszewski Ryszard
Department of Dermatology, Venereology and Allergology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland.
Laboratory of Molecular Basis of Synaptic Plasticity, Centre of New Technologies, University of Warsaw, Warsaw, Poland.
Postepy Dermatol Alergol. 2023 Jun;40(3):390-397. doi: 10.5114/ada.2023.128978. Epub 2023 Jul 15.
Chronic wounds are an increasing problem for health care all over the world. New treatment options for this illness are desired, especially antimicrobial agents. Silver nanoparticles (AgNPs) can be a potential substance that may be used in treatment of chronic wounds due to the growing antibiotic resistance.
To synthetize silver nanoparticles that are stable, pure and effective against bacteria.
The synthesis was conducted with chemical methods using different coating factors. The antistaphylococcal properties were analysed with the microdilution method to determine minimal inhibition concentrations (MIC) value. AgNPs were purified by dialysis. Moreover, keratinocyte cytotoxic properties of AgNPs were also assessed.
A method of synthesizing stable and efficient AgNPs has been developed. The type of the coating substance has a significant effect on AgNPs antimicrobial properties. Most of the silver nanoparticles, synthesized based on literature data, turned out to be durable during a few hours. This study has proven that depending on the coating factor, AgNPs stability ranges from 4 weeks to even 12 months. Unfortunately, the type of the stabilizer used also affects the cytotoxicity of AgNPs. It has been shown that dialysis is a substance purification method that is cheap, simple and easy to apply when dealing with high volume solutions.
AgNPs could be an alternative to widely used antibiotics and disinfectants. Nevertheless, the introduction of those substances to health care requires detailed long-term research not only in the field of safe use, yet also durability and purity of AgNPs solutions used.
慢性伤口在全球医疗保健领域正成为一个日益严重的问题。人们期望获得针对这种疾病的新治疗选择，尤其是抗菌剂。由于抗生素耐药性不断增加，银纳米颗粒（AgNP）可能是一种可用于治疗慢性伤口的潜在物质。
合成稳定、纯净且对细菌有效的银纳米颗粒。
采用化学方法并使用不同的包覆因子进行合成。用微量稀释法分析抗葡萄球菌特性以确定最低抑菌浓度（MIC）值。通过透析对AgNP进行纯化。此外，还评估了AgNP对角质形成细胞的细胞毒性特性。
已开发出一种合成稳定且高效的AgNP的方法。包覆物质的类型对AgNP的抗菌特性有显著影响。根据文献数据合成的大多数银纳米颗粒在数小时内是稳定的。本研究证明，根据包覆因子的不同（这里似乎少了一些关于包覆因子不同导致稳定性不同的具体阐述，原文表述不太完整），AgNP的稳定性范围从4周至甚至12个月。不幸的是，所使用的稳定剂类型也会影响AgNP的细胞毒性。结果表明，透析是一种在处理大量溶液时廉价、简单且易于应用的物质纯化方法。
AgNP可能成为广泛使用的抗生素和消毒剂的替代品。然而，将这些物质引入医疗保健领域不仅需要在安全使用方面进行详细的长期研究，还需要对所使用的AgNP溶液的耐久性和纯度进行研究。
