Elfadadny Ahmed, Uchiyama Jumpei, Goto Kazuyoshi, Imanishi Ichiro, Ragab Rokaia F, Nageeb Wedad M, Iyori Keita, Toyoda Yoichi, Tsukui Toshihiro, Ide Kaori, Kawamoto Keiko, Nishifuji Koji
Laboratory of Internal Medicine, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Department of Animal Internal Medicine, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt.
Front Vet Sci. 2023 Jul 20;10:1074127. doi: 10.3389/fvets.2023.1074127. eCollection 2023.
The strong bond between dogs and their owners creates a close association that could result in the transfer of antibiotic-resistant bacteria from canines to humans, potentially leading to the spread of antimicrobial resistance genes. , a common causative agent of persistent ear infections in dogs, is often resistant to multiple antibiotics. Assessing the antimicrobial resistance profile and genotype of is crucial for the appropriate use of veterinary pharmaceuticals. However, in recent years, few studies have been conducted on this bacterium in Japan. We determined the antimicrobial resistance profile and genotype of isolated from the ear canal of dogs in Japan in 2020. Analysis of antimicrobial resistance using disk diffusion tests indicated a high frequency of resistance to most antimicrobial agents. Particularly, 29 isolates from the ear canals of the 29 affected dogs (100%) were resistant to cefovecin, cefpodoxime, and florfenicol; however, they were susceptible to cefepime and piperacillin/tazobactam. Only 3.4, 10.3, and 10.3% of the isolates were resistant to ceftazidime, tobramycin, and gentamicin, respectively. Furthermore, upon analyzing the population structure using multilocus sequence typing, a considerably large clonal complex was not observed in the tested isolates. Three isolates, namely ST3881, ST1646, and ST532, were clonally related to the clinically isolated sequence types in Japan (such as ST1831, ST1413, ST1812, and ST1849), which is indicative of dog-to-human transmission. Considering the variation in antibiotic resistance compared to that reported by previous studies and the potential risk of dog-to-human transmission, we believe that the survey for antimicrobial resistance profile and population structure should be continued regularly. However, the prevalence of multidrug-resistant in dogs in Japan is not a crisis.
狗与其主人之间的紧密联系形成了一种密切关联，这可能导致抗生素耐药菌从犬类传播至人类，进而有可能致使抗菌药物耐药基因扩散。犬中耳炎常见的病原体，通常对多种抗生素具有耐药性。评估该菌的抗菌药物耐药谱和基因型对于合理使用兽用药物至关重要。然而，近年来日本针对这种细菌开展的研究较少。我们测定了2020年从日本犬类耳道分离出的该菌的抗菌药物耐药谱和基因型。采用纸片扩散法进行抗菌药物耐药性分析表明，大多数抗菌药物的耐药频率较高。特别是，从29只患病犬的耳道中分离出的29株菌株（100%）对头孢维星、头孢泊肟和氟苯尼考耐药；然而，它们对头孢吡肟和哌拉西林/他唑巴坦敏感。分别只有3.4%、10.3%和10.3%的分离株对头孢他啶、妥布霉素和庆大霉素耐药。此外，通过多位点序列分型分析群体结构时，在测试的分离株中未观察到相当大的克隆复合体。三个分离株，即ST3881、ST1646和ST532，与日本临床分离的序列类型（如ST1831、ST1413、ST1812和ST1849）存在克隆相关性，这表明存在犬传人现象。考虑到与先前研究报道相比抗生素耐药性的变化以及犬传人潜在风险，我们认为应定期持续开展抗菌药物耐药谱和群体结构调查。然而，日本犬类中多重耐药该菌的流行情况并非危机。
