Al-Ghanayem Abdullah A
Department of Clinical Laboratory Science, College of Applied Medical Sciences, Shaqra University, Shaqra, Saudi Arabia.
Front Pharmacol. 2024 Oct 3;15:1388625. doi: 10.3389/fphar.2024.1388625. eCollection 2024.
Acne vulgaris is a skin infection widely seen in adolescents between 10-19 years with males affected more than females. It mainly affects the face but may also affect the back and chest. The symptoms vary with mild acne manifesting as comedones and moderate acne as inflammatory lesions (papulopustular), nodules, and mild scarring while severe acne has the same symptoms that have not subsided within 6 months of treatment. Various treatments including topical medications containing different antibiotics are used to treat acne. Recently, herbal treatments have been shown as better alternatives to conventional treatment. Ging. ex Benth () is traditionally used for skin infections such as wound healing and biofilm formation.
Methanolic extract of was subjected to liquid chromatography-mass spectrometry (LC-MS) analysis, and its antibacterial effect against . The anti-acne, anti-inflammatory, and antioxidant effects were also assessed using HaCaT cells infected with The cytotoxicity of the extract was evaluated using a neutral red uptake assay, and anti-inflammatory effects were determined by measuring TNF-α, IL-1β, INF-γ, and COX2 inhibition. The antioxidant action was assessed by ROS generation in HaCaT cells infected with .
LC-MS analysis of the extract showed the presence of 16 different metabolites with L-carnitine, esculin sesquihydrate, and gamma-linoleic acid as major metabolites. The methanolic extract of showed an antibacterial effect against with an IC value of 263.2 μg/mL. The extract attenuated the cytotoxicity of on the HaCaT cell and the IC was found to be 676.2 μg/mL. It also decreased dose-dependently the expression of TNF-α, IL-1β, INF-γ, and inhibited COX2 in the HaCaT cells infected with . It also decreased the generation of reactive oxygen species.
The results support the use of as an anti-acne agent but it possesses mild antibacterial action. It showed anti-inflammatory effects in HaCaT cells infected with . It is also an effective antioxidant and decreased the generation of reactive oxygen species. Comparison of the anti-acne effects and adverse reactions of extract with other treatments will provide more insight into its clinical efficacy and toxicity.
寻常痤疮是一种在10至19岁青少年中广泛可见的皮肤感染疾病，男性患病率高于女性。它主要影响面部，但也可能影响背部和胸部。症状各不相同，轻度痤疮表现为粉刺，中度痤疮表现为炎性皮损（丘疹脓疱）、结节和轻度瘢痕形成，而重度痤疮则是在治疗6个月内症状仍未消退。包括含有不同抗生素的外用药物在内的各种治疗方法都用于治疗痤疮。最近，草药治疗已被证明是传统治疗的更好替代方法。 传统上用于伤口愈合和生物膜形成等皮肤感染。
对 的甲醇提取物进行液相色谱 - 质谱（LC - MS）分析，并检测其对 的抗菌作用。还使用感染了 的HaCaT细胞评估其抗痤疮、抗炎和抗氧化作用。使用中性红摄取试验评估提取物的细胞毒性，并通过测量TNF - α、IL - 1β、INF - γ和COX2抑制来确定抗炎作用。通过感染了 的HaCaT细胞中活性氧的产生来评估抗氧化作用。
提取物的LC - MS分析显示存在16种不同的代谢物，其中L - 肉碱、七水合七叶苷和γ - 亚油酸为主要代谢物。 的甲醇提取物对 显示出抗菌作用，IC值为263.2μg/mL。提取物减轻了 对HaCaT细胞的细胞毒性，IC值为676.2μg/mL。它还在感染了 的HaCaT细胞中剂量依赖性地降低TNF - α、IL - 1β、INF - γ的表达并抑制COX2。它还减少了活性氧的产生。
结果支持将 用作抗痤疮药物，但它具有轻度抗菌作用。它在感染了 的HaCaT细胞中显示出抗炎作用。它也是一种有效的抗氧化剂，减少了活性氧的产生。将提取物的抗痤疮作用和不良反应与其他治疗方法进行比较，将更深入了解其临床疗效和毒性。
