Rizwan Muhammad, Faisal Shah, Tariq Muhammad Hamza, Zafar Sania, Khan Ajmal, Ahmad Farhan
Center for Biotechnology and Microbiology, University of Swat, Swat 19120, Khyber Pakhtunkhwa, Pakistan.
Institute of Biotechnology and Microbiology, Bacha Khan University, Charsadda 24460, Khyber Pakhtunkhwa, Pakistan.
ACS Omega. 2023 Jul 17;8(30):27439-27449. doi: 10.1021/acsomega.3c02932. eCollection 2023 Aug 1.
Nanotechnology is a research hotspot that has gained considerable interest due to its potential inferences in the bioscience, medical, and engineering disciplines. The present study uses biomass from the EAF63 strain to create bio-inspired metallic tin oxide nanoparticles (SnO NPs). The biosynthesized NPs were extensively analyzed using UV spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and Fourier transform infrared (FTIR) techniques. The identification of the crystalline phase was confirmed by XRD. The SEM technique elucidated the morphological characteristics and size of SnO NPs. SEM investigation revealed that the SnO NPs have a size of 10 nm with spherical morphology. The capping of NPs was confirmed by FTIR analysis that revealed the presence of different compounds found in the biomass of the EAF63 strain. Later, EDX confirmed the elemental composition of NPs. Moreover, the synthesized SnO NPs were employed for important applications including anti-aging, anti-Alzheimer's, anti-inflammatory, anti-larvicidal, and antibacterial action against sinusitis pathogens. The highest value was observed for (19.75 ± 0.46), followed by (17.49 ± 0.82) and (15.31 ± 0.73), respectively. Among the used concentrations, the highest inhibition of 76.8 ± 0.93 for 15-lipoxygenase (15-LOX) was observed at 400 μg/mL, followed by 67.4 ± 0.91 for cyclooxygenase-1 (COX-1). So, as an outcome, -mediated SnO NPs might be considered as the safe and effective nanoplatforms for multifunctional biological applications in the field of nanomedicine.
纳米技术是一个研究热点，因其在生物科学、医学和工程学科中的潜在影响而备受关注。本研究利用EAF63菌株的生物质来制备受生物启发的金属氧化锡纳米颗粒（SnO NPs）。使用紫外光谱、X射线衍射（XRD）、扫描电子显微镜（SEM）、能量色散X射线光谱（EDX）和傅里叶变换红外（FTIR）技术对生物合成的纳米颗粒进行了广泛分析。通过XRD确认了晶相。SEM技术阐明了SnO NPs的形态特征和尺寸。SEM研究表明，SnO NPs的尺寸为10 nm，呈球形形态。通过FTIR分析证实了纳米颗粒的封端，该分析揭示了EAF63菌株生物质中存在的不同化合物。随后，EDX确认了纳米颗粒的元素组成。此外，合成的SnO NPs被用于重要应用，包括抗衰老、抗阿尔茨海默病、抗炎、抗杀幼虫以及对鼻窦炎病原体的抗菌作用。观察到的最高值为（19.75±0.46），其次分别为（17.49±0.82）和（15.31±0.73）。在所使用的浓度中，在400μg/mL时观察到对15-脂氧合酶（15-LOX）的最高抑制率为76.8±0.93，其次是对环氧合酶-1（COX-1）的抑制率为67.4±0.91。因此，作为一个结果，介导的SnO NPs可能被认为是纳米医学领域多功能生物应用的安全有效的纳米平台。
