Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria 21568, Egypt.
Chemistry Department, Faculty of Science, Alexandria University, Alexandria 21568, Egypt.
Int J Mol Sci. 2022 Sep 6;23(18):10243. doi: 10.3390/ijms231810243.
Biofilms are matrix-enclosed communities of bacteria that are highly resistant to antibiotics. Adding nanomaterials with antibacterial activity to the implant surfaces may be a great solution against biofilm formation. Due to its potent and widespread antibacterial effect, silver nanoparticles were considered the most potent agent with different biological activities. In the present investigation, silver nanoparticles (AgNPs) were newly synthesized as antibiofilm agents using sugarcane process byproduct (molasses) and named Mo-capped AgNPs. The synthesized nanoparticles showed promising antimicrobial activity against ATCC 6538 and DAY185. Statistically designed optimization through response surface methodology was evaluated for maximum activity and better physical characteristics, namely the nanoparticles' size and polydispersity index (PDI), and it was revealed that molasses concentration was the main effective factor. Minimal biofilm eradication concentration (MBEC) of Mo-capped AgNPs against ATCC 6538 and DAY185 was 16 and 32 µg/mL, respectively. Scanning electron microscope study of Mo-capped AgNP-treated biofilm revealed that AgNPs penetrated the preformed biofilm and eradicated the microbial cells. The optimally synthesized Mo-capped AgNPs were spherically shaped, and the average size diameter ranged between 29 and 88 nm with high proportions of Ag element (78.0%) recorded. Fourier-transform infrared spectroscopy (FTIR) analysis indicated the importance of molasses ingredients in capping and stabilizing the produced silver nanoparticles.
生物膜是由细菌组成的基质封闭群落，对抗生素具有高度抗性。在植入物表面添加具有抗菌活性的纳米材料可能是对抗生物膜形成的绝佳解决方案。由于其强大而广泛的抗菌作用，银纳米颗粒被认为是具有不同生物学活性的最有效试剂。在本研究中，银纳米颗粒（AgNPs）是使用甘蔗加工副产物（糖蜜）作为新的生物膜抑制剂通过糖蜜法合成的，并命名为 Mo 封端的 AgNPs。合成的纳米颗粒对 ATCC 6538 和 DAY185 表现出有希望的抗菌活性。通过响应面法进行统计学设计优化，以评估最大活性和更好的物理特性，即纳米颗粒的尺寸和多分散指数（PDI），结果表明糖蜜浓度是主要的有效因素。Mo 封端的 AgNPs 对 ATCC 6538 和 DAY185 的最小生物膜消除浓度（MBEC）分别为 16 和 32 μg/mL。用 Mo 封端的 AgNP 处理生物膜的扫描电子显微镜研究表明，AgNPs 穿透了已形成的生物膜并消灭了微生物细胞。最佳合成的 Mo 封端的 AgNPs 呈球形，平均粒径范围在 29 至 88nm 之间，Ag 元素的比例很高（78.0%）。傅里叶变换红外光谱（FTIR）分析表明，糖蜜成分在封端和稳定所产生的银纳米颗粒中具有重要作用。
