Voblikova Tatiana, Laricheva Kristina
Yaroslav-the-Wise Novgorod State University, 173003 Veliky Novgorod, Russia.
Int J Food Sci. 2023 Jul 27;2023:8484835. doi: 10.1155/2023/8484835. eCollection 2023.
The use of an alginate hydrogel exclusively for the immobilization of bifidobacteria during oral delivery led to a decrease in the total number of bifidobacteria to 4.0 lg CFU/ml in pH gradients in models of the stomach and intestines, which required clarification of the composition of the protective coating. The introduction of resistant starch into the composition of the microcapsule contributed to the preservation of the viability of immobilized bifidobacteria up to 87% of the initial concentration when passing through the model environment simulating the human digestion system. The introduction of sodium carboxymethylcellulose into the composition of the hydrogel contributed to the regulation of the degradation of the polymer matrix and the controlled release of bifidobacteria. The use of sodium carboxymethylcellulose 0.5% in the formation of a polymer microcapsule provided the maximum encapsulation efficiency of 93.2% and the maximum decay rate of bacteria-loaded microcapsules of 95.7%. The modified alginate matrix contributes to maintaining the level of viable cells of probiotic microorganisms ( 791) of at least 10 CFU/g when stored for three weeks. As a result of the research, a system for oral delivery of immobilized bifidobacteria in the structure of microparticles with a closed surface in the matrix of a milk drink has been developed, which increases the effectiveness of probiotics for human health in the composition of food products.
仅在口服给药过程中使用藻酸盐水凝胶固定双歧杆菌，在胃和肠道模型的pH梯度中，双歧杆菌总数降至4.0 lg CFU/ml，这需要对保护涂层的组成进行澄清。在微胶囊组成中引入抗性淀粉，有助于在通过模拟人体消化系统的模型环境时，使固定化双歧杆菌的存活率保持在初始浓度的87%。在水凝胶组成中引入羧甲基纤维素钠有助于调节聚合物基质的降解和双歧杆菌的控释。在形成聚合物微胶囊时使用0.5%的羧甲基纤维素钠可提供93.2%的最大包封效率和95.7%的载菌微胶囊最大衰减率。改性藻酸盐基质有助于在储存三周时将益生菌微生物（791）的活细胞水平维持在至少10 CFU/g。研究结果表明，已开发出一种在乳饮料基质中以具有封闭表面的微粒结构口服递送固定化双歧杆菌的系统，该系统提高了食品成分中益生菌对人体健康的功效。
