Wang Mengmeng, Zang Yipeng, Hong Kangjin, Zhao Xiaofeng, Yu Chenrui, Liu Dandan, An Zichao, Wang Liyuan, Yue Wenjin, Nie Guangjun
College of Biological and Food Engineering, Anhui Polytechnic University, 241000 Wuhu, China.
Institute of Developmental and Regenerative Biology, Zhejiang Key Laboratory of Organ Development and Regeneration, 310036 Hangzhou, China; College of Life and Environmental Sciences, Hangzhou Normal University, 310036 Hangzhou, China.
Int J Biol Macromol. 2021 Dec 1;192:684-691. doi: 10.1016/j.ijbiomac.2021.10.019. Epub 2021 Oct 12.
pH-sensitive hydrogels have been applied in delivering probiotics and drugs. However, pH sensitivity has been found to be contradictory with structural stability in hydrogel preparation. In this work, a novel strategy based on two systems of sodium carboxymethyl cellulose (CMC)/chitosan (CS) and sodium alginate (SA)/calcium chloride was designed to construct a reticulated shell structure stable for 3 h in simulated gastric fluid (pH 1.2) but began to break up at 2 h in simulated intestinal fluid (pH 6.8), exhibiting obvious pH sensitivity. The embedding rate of Bacillus subtilis natto reached to 67.3%, and the sustained release lasted for more than 10 h. It is implicated that the reticulated shell structure has harmoniously balanced the two incompatible properties of pH sensitivity and sustained release of CMC/CS/SA beads.
pH敏感水凝胶已应用于益生菌和药物递送。然而，在水凝胶制备过程中，人们发现pH敏感性与结构稳定性相互矛盾。在这项工作中，设计了一种基于羧甲基纤维素钠（CMC）/壳聚糖（CS）和海藻酸钠（SA）/氯化钙两个体系的新策略，以构建一种网状壳结构，该结构在模拟胃液（pH 1.2）中稳定3小时，但在模拟肠液（pH 6.8）中2小时后开始分解，表现出明显的pH敏感性。纳豆芽孢杆菌的包埋率达到67.3%，缓释持续超过10小时。这表明网状壳结构已和谐地平衡了CMC/CS/SA微珠pH敏感性和缓释这两个不相容的特性。
