Department of Oncology, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
Department of Emergency Internal Medicine, The First Affiliated Hospital of Wannan Medical College Yijishan Hospital, Wuhu, China.
PeerJ. 2023 Jul 31;11:e15774. doi: 10.7717/peerj.15774. eCollection 2023.
To investigate the expression and correlation of COX-2 and NUCB1 in colorectal adenocarcinoma and adjacent tissues.
The expression of COX-2 and NUCB1 and their effects on prognosis were predicted using bioinformatics. Immunohistochemistry was used to identify the expression of two molecules in 56 cases of colorectal adenocarcinoma and the surrounding tissues. The expression of two molecules and their association with clinicopathological variables were examined using the chi-square test. The association between COX-2 and NUCB1 was investigated using the Spearman correlation test.
The STRING database revealed that COX-2 and NUCB1 were strongly linked. According to the UALCAN and HPA database, COX-2 was upregulated while NUCB1 was downregulated in colorectal adenocarcinoma, both at the protein and gene levels. The OS times for COX-2 and NUCB1 high expression, however, exhibited the same patterns. The rate of positive COX-2 immunohistochemical staining in cancer tissues was 69.64% (39/56), which was significantly higher than the rate in healthy tissues 28.57% (16/56). NUCB1 was expressed positively in cancer tissues at a rate of 64.29% (36/56) compared to just 19.64% (11/56) in neighboring tissues. The positive expression levels of COX-2 and NUCB1 were both closely related to clinical stage, differentiation degree, and lymphatic metastases ( < 0.05). In colorectal cancer, COX-2 and NUCB1 expression were significantly correlated (r = 0.6312, < 0.001).
Both COX-2 and NUCB1 are overexpressed and significantly associated in colorectal adenocarcinoma.
研究 COX-2 和 NUCB1 在结直肠腺癌及相邻组织中的表达及相关性。
利用生物信息学预测 COX-2 和 NUCB1 的表达及其对预后的影响。采用免疫组织化学法检测 56 例结直肠腺癌及周围组织中两种分子的表达。采用卡方检验检测两种分子的表达及其与临床病理变量的关系。采用 Spearman 相关检验分析 COX-2 与 NUCB1 的相关性。
STRING 数据库显示 COX-2 和 NUCB1 之间存在强关联。根据 UALCAN 和 HPA 数据库，COX-2 在结直肠腺癌中呈高表达，而 NUCB1 呈低表达，蛋白和基因水平均如此。然而，COX-2 和 NUCB1 高表达的 OS 时间却呈现相同的模式。癌组织中 COX-2 免疫组化染色阳性率为 69.64%（39/56），明显高于癌旁组织的 28.57%（16/56）。NUCB1 在癌组织中的阳性表达率为 64.29%（36/56），而在癌旁组织中仅为 19.64%（11/56）。COX-2 和 NUCB1 的阳性表达水平均与临床分期、分化程度和淋巴转移密切相关（<0.05）。在结直肠癌中，COX-2 和 NUCB1 的表达呈显著正相关（r=0.6312，<0.001）。
COX-2 和 NUCB1 在结直肠腺癌中均呈高表达，且表达显著相关。
