Department of Biochemical Sciences, Sapienza University of Rome, 00185, Rome, Italy.
Department of Biochemical Sciences, Sapienza University of Rome, 00185, Rome, Italy; Department of Brain Sciences, Imperial College London, London, W12 0NN, United Kingdom.
Arch Biochem Biophys. 2023 Sep 1;745:109714. doi: 10.1016/j.abb.2023.109714. Epub 2023 Aug 5.
RNA is a fundamental nucleic acid for life and it plays important roles in the regulation of gene transcription, post-transcriptional regulation, and epigenetic regulation. Recently, the focus on this nucleic acid has significantly increased due to the development of mRNA vaccines and RNA-based gene therapy protocols. Unfortunately, RNA based products show constrains mainly owing to instability and easy degradability of the RNA molecules. Indeed, unlike the DNA molecule which has a great intrinsic stability, RNA is more prone to degradation and this process is accelerated under thermal treatment. Here we describe a method that involves the use of Natural Deep Eutectic Solvents (NaDES) capable of slowing down RNA degradation process. Our results show that this technology seems suitable for improving the stability of specific RNA molecules particularly susceptible to thermal-induced degradation. Therefore, this technique represents a valuable tool to stabilize RNA molecules used in gene therapy and mRNA vaccines.
RNA 是生命的基本核酸，在基因转录调控、转录后调控和表观遗传调控中发挥重要作用。最近，由于 mRNA 疫苗和基于 RNA 的基因治疗方案的发展，人们对这种核酸的关注度显著提高。不幸的是，基于 RNA 的产品显示出一定的局限性，主要是由于 RNA 分子的不稳定性和易降解性。事实上，与具有内在高稳定性的 DNA 分子不同，RNA 更容易降解，并且在热处理下，这个过程会加速。在这里，我们描述了一种涉及使用天然深共熔溶剂（NaDES）的方法，该方法能够减缓 RNA 降解过程。我们的结果表明，这项技术似乎适合提高对热诱导降解特别敏感的特定 RNA 分子的稳定性。因此，该技术代表了一种稳定用于基因治疗和 mRNA 疫苗的 RNA 分子的有价值的工具。
