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Expression of a mutant vasopressin gene: differential polyadenylation and read-through of the mRNA 3' end in a frame-shift mutant.

作者信息

Ivell R, Schmale H, Krisch B, Nahke P, Richter D

出版信息

EMBO J. 1986 May;5(5):971-7. doi: 10.1002/j.1460-2075.1986.tb04311.x.

DOI:10.1002/j.1460-2075.1986.tb04311.x
PMID:3755103
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1166890/
Abstract

Sequence analysis of cDNA clones derived from hypothalamic mRNA of diabetes insipidus (Brattleboro) rats shows that the vasopressin gene transcript also includes the single base deletion demonstrated in the gene. This causes a frame-shift in the C terminus of the vasopressin precursor with a reading frame open through the 3' end of the mRNA including the poly(A) sequence. Antibodies raised against a synthetic tetradecapeptide (CP-14) corresponding to the frame-shifted C terminus identified a product of mol. wt approximately 26 000 in a reticulocyte lysate system programmed with Brattleboro hypothalamic mRNA. Immunohistochemical analysis indicated that a similar precursor is also present in vivo in neurones of the Brattleboro hypothalamus. Electrophoretic analysis of vasopressin mRNA from wild-type and mutant rat tissues revealed that (i) the hypothalamic mRNA from Brattleboro rats contains a longer stretch of poly(A) sequence than the wild-type strains; (ii) vasopressin mRNA is also present in the adrenal, ovary, testis and cerebellum, at very low levels; however, (iii) the extra-hypothalamic mRNA is considerably shorter than that in the hypothalamus because of a curtailed poly(A) sequence. Thus similar vasopressin gene transcripts are subject to a tissue-specific differential polyadenylation.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/7030723aa4ef/emboj00168-0154-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/1b647108030d/emboj00168-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/3bfaa8ae669e/emboj00168-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/32fb3aff8801/emboj00168-0153-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/66928f626536/emboj00168-0153-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/2bab4b53e8f3/emboj00168-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/7030723aa4ef/emboj00168-0154-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/1b647108030d/emboj00168-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/3bfaa8ae669e/emboj00168-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/32fb3aff8801/emboj00168-0153-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/66928f626536/emboj00168-0153-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/2bab4b53e8f3/emboj00168-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/1166890/7030723aa4ef/emboj00168-0154-b.jpg

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1
Expression of a mutant vasopressin gene: differential polyadenylation and read-through of the mRNA 3' end in a frame-shift mutant.
EMBO J. 1986 May;5(5):971-7. doi: 10.1002/j.1460-2075.1986.tb04311.x.
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本文引用的文献

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Regul Pept. 1984 Mar;8(2):141-6. doi: 10.1016/0167-0115(84)90169-1.
2
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Nature. 1984;308(5961):705-9. doi: 10.1038/308705a0.
3
The mutant vasopressin gene from diabetes insipidus (Brattleboro) rats is transcribed but the message is not efficiently translated.
下丘脑 - 垂体 - 肾上腺轴调节的遗传学方法
Neuropsychopharmacology. 2016 Jan;41(1):245-60. doi: 10.1038/npp.2015.215. Epub 2015 Jul 20.
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Cellular and subcellular evidence for neuronal interaction between the chemokine stromal cell-derived factor-1/CXCL 12 and vasopressin: regulation in the hypothalamo-neurohypophysial system of the Brattleboro rats.趋化因子基质细胞衍生因子-1/CXCL 12与血管加压素之间神经元相互作用的细胞及亚细胞证据：对布拉特洛维大鼠下丘脑-神经垂体系统的调节
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