Bioactive Molecules Research Laboratory, Doctoral School of Sciences and Technologies, Faculty of Sciences II, Lebanese University, Fanar, Lebanon.
Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), EA 4492, SFR Condorcet FR CNRS 3417, Université du Littoral-Côte d'Opale (ULCO), 59140 Dunkerque, France.
Molecules. 2018 May 17;23(5):1204. doi: 10.3390/molecules23051204.
Cyclodextrins (CDs) are a family of cyclic oligosaccharides that constitute one of the most widely used molecular hosts in supramolecular chemistry. Encapsulation in the hydrophobic cavity of CDs positively affects the physical and chemical characteristics of the guests upon the formation of inclusion complexes. Such a property is interestingly employed to retain volatile guests and reduce their volatility. Within this scope, the starting crucial point for a suitable and careful characterization of an inclusion complex is to assess the value of the formation constant (K), also called stability or binding constant. This task requires the application of the appropriate analytical method and technique. Thus, the aim of the present paper is to give a general overview of the main analytical tools used for the determination of K values for CD/volatile inclusion complexes. This review emphasizes on the advantages, inconvenients and limits of each applied method. A special attention is also dedicated to the improvement of the current methods and to the development of new techniques. Further, the applicability of each technique is illustrated by a summary of data obtained from the literature.
环糊精（CDs）是一类环状低聚糖，是超分子化学中应用最广泛的分子主体之一。客体分子包合在 CD 的疏水性空腔内，形成包合物后会显著影响客体的物理和化学性质。这种性质可用于保留挥发性客体并降低其挥发性。在这一领域中，对包含物进行合适且仔细的特征描述的起始关键点是评估形成常数（K）的值，也称为稳定性或结合常数。此任务需要应用适当的分析方法和技术。因此，本文的目的是综述用于测定 CD/挥发性包含物形成常数（K）的主要分析工具。本文重点介绍了每种应用方法的优缺点和局限性。特别关注当前方法的改进和新技术的开发。此外，还通过文献中获得的数据总结说明了每种技术的适用性。
