Cooper N G, Steindler D A
J Comp Neurol. 1986 Jul 8;249(2):157-69. doi: 10.1002/cne.902490204.
Plant lectins were used to examine the disposition of glycosylated molecules in vibratome sections through the barrel subfield of mouse somatosensory cortex at selected times during postnatal development. The peroxidase conjugates of peanut agglutinin (PNA, specific for N-acetylgalactosamine), concanavalin A (specific for mannose), and wheat germ agglutinin (specific for N-acetylglucosamine and N-acetylneuraminic acid) were used to study lectin binding in aldehyde-fixed tissue sections of cortex. Following peroxidase cytochemistry and light microscopy, it was found that all three lectins bound in the region of the barrel subfield as early as postnatal day 3 (day of birth = postnatal day 1). The lectins bound to the prospective sides and/or septae of individual barrels in preference to the prospective hollows. This lectin demarcation of the barrel field occurred prior to the detection of this region with cresyl violet staining and was still demonstrable on postnatal day 6, when the individual barrels became discernible with cresyl violet. This suggests that the lectin binding material is present before the barrel field becomes a fully formed and organized region. A decrease in lectin affinity for binding sites in these tissue sections occurs during postnatal development (Cooper and Steindler: Soc. Neurosci. (Abstr.) 10: 43a, '84) and this study demonstrates that lectins do not delineate the barrel field of more mature animals (2-3 months old), whereas barrels can be detected with cresyl violet at this time. A preliminary electron microscope analysis of the postnatal day 6 somatosensory cortex demonstrates that the lectin PNA binds to elements of the forming neuropil and also to Golgi apparatus intermediate saccules in neuronal cells. The prospective barrel field can be detected with lectins during a critical period in development in which alterations can occur in the barrel field in response to peripheral deprivation (Jeanmonod et al: Neuroscience 6:1503-35, '81) and therefore we suggest that the glycans visualized with lectin-peroxidase conjugates denote possible candidates for molecules involved in shaping barrel structure.
在出生后发育的特定时间，利用植物凝集素检测振动切片中糖基化分子在小鼠体感皮层桶状亚区的分布情况。使用花生凝集素（PNA，对N - 乙酰半乳糖胺具有特异性）、伴刀豆球蛋白A（对甘露糖具有特异性）和小麦胚凝集素（对N - 乙酰葡糖胺和N - 乙酰神经氨酸具有特异性）的过氧化物酶偶联物，研究凝集素在皮层醛固定组织切片中的结合情况。经过过氧化物酶细胞化学和光学显微镜观察，发现早在出生后第3天（出生日 = 出生后第1天），所有三种凝集素就在桶状亚区区域结合。凝集素优先结合单个桶的预期侧面和/或隔膜，而非预期的空洞。在通过甲酚紫染色检测到该区域之前，凝集素就对桶状区域进行了划分，并且在出生后第6天仍然可以看到，此时用甲酚紫可以分辨出单个桶。这表明在桶状区域成为一个完全形成且有组织的区域之前，凝集素结合物质就已存在。在出生后发育过程中，这些组织切片中凝集素与结合位点的亲和力会降低（库珀和施泰德勒：《神经科学学会会刊》（摘要）10: 43a，'84），本研究表明凝集素无法描绘更成熟动物（2 - 3个月大）的桶状区域，而此时用甲酚紫可以检测到桶状结构。对出生后第6天的体感皮层进行的初步电子显微镜分析表明，凝集素PNA与正在形成的神经毡成分以及神经元细胞中的高尔基体中间囊泡结合。在发育的关键时期，可以用凝集素检测到预期的桶状区域，在此期间，桶状区域可能会因外周剥夺而发生改变（让莫诺德等人：《神经科学》6:1503 - 35，'81），因此我们认为用凝集素 - 过氧化物酶偶联物可视化的聚糖代表了参与塑造桶状结构的分子的可能候选物。
