Bryan-Thomas Joneshia, McClear Teena, Omoregie Samson
Department of Medical Technology, Northern Caribbean University, Mandeville, Jamaica.
Department of Biology, Chemistry and Environmental Science, Northern Caribbean University, Mandeville, Jamaica.
Saudi J Biol Sci. 2023 Sep;30(9):103749. doi: 10.1016/j.sjbs.2023.103749. Epub 2023 Jul 28.
Garlic () is generally known to be of medicinal value, possessing potentials that include antimicrobial activity, but are often consumed in foods after subjection to cooking heat. The antimicrobial potential of heat stressed garlic may become decreased or lost when cooked, making its medicinal benefit unavailable to consumers. The potential of uncooked and cooked extracts from garlic imported to Jamaica, to inhibit the growth of eight microbes of clinical significance was investigated. Aqueous extracts of fresh garlic of 15 g/100 ml (fw), and dried and pulverized garlic cloves of 12.5 g/100 ml, 25 g/100 ml, 50 g/100 ml, and 100 g/100 ml (dw), were tested for inhibition of microbial growth. Extracts were tested uncooked, and cooked by boiling for 5, 10, and 15 min respectively. Of all the microbes studied, incurred the largest zone of inhibition (57.7 ± 0.6 mm at the 100 g/100 ml of the dried extract, (3, 8) = 51.778, < 0.001, ω = 0.93). Cooking of garlic extracts resulted in statistically significant decreases in zones of inhibition of microbes, as evident in the linear regression and one-way ANOVA analyses, and/or complete loss of microbial inhibition. was the most inhibited microbe, followed by , and sp., respectively. The use of uncooked garlic may be the best route for obtaining the greatest antimicrobial potential of garlic against susceptible bacteria and fungi because cooking heat stress resulted in the decrease and complete loss of the antimicrobial potentials of the garlic.
大蒜()通常被认为具有药用价值,具有包括抗菌活性在内的多种潜能,但经过烹饪加热后常被用于食品中。受热应激的大蒜的抗菌潜能在烹饪时可能会降低或丧失,从而使消费者无法获得其药用益处。对进口到牙买加的生蒜和熟蒜提取物抑制8种具有临床意义的微生物生长的潜能进行了研究。测试了15 g/100 ml(鲜重)的新鲜大蒜水提取物,以及12.5 g/100 ml、25 g/100 ml、50 g/100 ml和100 g/100 ml(干重)的干燥和粉碎蒜瓣提取物对微生物生长的抑制作用。提取物分别进行了未煮、煮5分钟、10分钟和15分钟的测试。在所有研究的微生物中,(在100 g/100 ml干提取物中抑菌圈最大,为57.7±0.6 mm,F(3, 8)=51.778,P<0.001,ω=0.93)。大蒜提取物的烹饪导致微生物抑菌圈在统计学上显著减小,这在线性回归和单因素方差分析中很明显,和/或微生物抑制作用完全丧失。是最易被抑制的微生物,其次分别是和sp.。使用生蒜可能是获得大蒜对易感细菌和真菌最大抗菌潜能的最佳途径,因为烹饪热应激导致大蒜抗菌潜能降低并完全丧失。