Microbial Oceanography Laboratory, School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, Seoul 08826, Republic of Korea.
Aquatic Disease Control Division, National Fishery Products Quality Management Service, Busan 46083, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Aug;73(8). doi: 10.1099/ijsem.0.005999.
A Gram-stain-negative, facultatively anaerobic, non-motile, rod-shaped bacterial strain, designated HL-MP18, was isolated from Arctic seawater after a prolonged incubation employing polypropylene as the sole carbon source. Phylogenetic analyses of the 16S rRNA gene sequence revealed that strain HL-MP18 was affiliated to the genus with close relatives LXJ103 (96.8 %) and KCTC 32327 (96.5 %). The complete genome sequence of strain HL-MP18 comprised a circular chromosome of 3.86 Mbp and two circular plasmids of 0.17 and 0.24 Mbp. Genomic comparisons based on average nucleotide identity and digital DNA-DNA hybridization showed that strain HL-MP18 was consistently discriminated from its closely related taxa in the genus . Strain HL-MP18 showed optimal growth at 25 °C, pH 7.0 and 2.5 % (w/v) sea salts. The major cellular fatty acids were C 6 and/or C 7 (49.6 %), C cyclo 8 (13.5 %), and C (12.8 %). The major respiratory quinone was ubiquinone-10. The polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid and three unidentified lipids. The genomic DNA G+C content of the strain was 59.2 mol%. The phylogenetic, genomic, phenotypic and chemotaxonomic results indicate that strain HL-MP18 is distinguishable from the recognized species of the genus . Therefore, we propose that strain HL-MP18 represents a novel species belonging to the genus , for which the name sp. nov. is proposed. The type strain is HL-MP18 (=KCCM 90405=JCM 35639).
一株革兰氏阴性、兼性厌氧、无动力、杆状细菌菌株，被命名为 HL-MP18，是从经过长时间培养的北极海水中分离出来的，使用聚丙烯作为唯一的碳源。16S rRNA 基因序列的系统发育分析表明，菌株 HL-MP18属于 属，与近缘种 LXJ103（96.8%）和 KCTC 32327（96.5%）最为相似。菌株 HL-MP18 的全基因组序列由一个 3.86 Mbp 的圆形染色体和两个 0.17 和 0.24 Mbp 的圆形质粒组成。基于平均核苷酸同一性和数字 DNA-DNA 杂交的基因组比较表明，菌株 HL-MP18与属内的近缘种始终存在差异。菌株 HL-MP18 在 25°C、pH 7.0 和 2.5%（w/v）海水盐度下生长最佳。主要的细胞脂肪酸为 C6 和/或 C7（49.6%）、C 环 8（13.5%）和 C（12.8%）。主要的呼吸醌为泛醌-10。极性脂类由磷脂酰胆碱、磷脂酰甘油、一种未鉴定的胺脂和三种未鉴定的脂类组成。菌株的基因组 DNA G+C 含量为 59.2mol%。系统发育、基因组、表型和化学分类学结果表明，菌株 HL-MP18与该属已鉴定的种有明显区别。因此，我们建议菌株 HL-MP18代表一个新的种，属于 属，建议命名为 sp. nov.。模式菌株为 HL-MP18（=KCCM 90405=JCM 35639）。
