Wei Qingcong, Ma Lin, Zhang Weiwei, Ma Guanglei, Hu Zhiguo
Collaborative Innovation Centre of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Engineering Research Centre of Chiral Hydroxyl Phar Henan Engineering Research Centre of Chiral Hydroxyl Pharmaceutical, Henan Engineering Laboratory of Chemical Pharmaceutical and Biomedical Materials, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, China.
J Mater Chem B. 2022 May 25;10(20):3927-3935. doi: 10.1039/d2tb00074a.
Hydrogel wound dressings have attracted intense and increasing interest for their extracellular matrix like properties and bioactive material delivery ability. Various functional hydrogels loaded with metals (and their oxides), antibiotics and anti-inflammatory agents have been prepared to realize antioxidant, bactericidal and anti-inflammatory effects, accelerating wound healing. Nevertheless, it is still a big challenge to facilely fabricate hydrogel wound dressings with inherent desirable properties to promote wound healing and avoid some drawbacks such as toxicity of metals and drug resistance. Herein, we facilely prepared a series of (-)-epigallocatechin-3--gallate (EGCG)-crosslinked carboxymethyl chitosan-based hydrogels (EP gels) with inherent antioxidant, bactericidal and adhesive properties. Gluconate-terminated polyethylene glycol (PEG-glu) was introduced into gel networks to enhance the mechanical properties. The hydrogels are constructed borate ester crosslinking between phenylboronic acid (PBA) groups of PBA-grafted carboxymethyl chitosan (CMCS-PBA) and diol groups of EGCG and PEG-glu. The hydrogels exhibited excellent self-healing properties, desirable mechanical and adhesive strength, free radical scavenging capability and outstanding bactericidal ability against and . In the subsequent full-thickness skin defect model of mice, EP1 gel could promote the proliferation and remodeling process such as the regeneration of epidermis, dermis, and skin appendages, deposition of collagen, and upregulation of the VEGF level, thereby accelerating the healing of damaged skin. Overall, we facilely prepared polysaccharide-based hydrogels with inherent desirable properties as promising dressings for wound repair.
水凝胶伤口敷料因其类似细胞外基质的特性和生物活性材料递送能力而引起了人们越来越浓厚的兴趣。已经制备了各种负载金属（及其氧化物）、抗生素和抗炎剂的功能性水凝胶，以实现抗氧化、杀菌和抗炎作用，加速伤口愈合。然而，轻松制备具有促进伤口愈合的固有理想特性并避免一些缺点（如金属毒性和耐药性）的水凝胶伤口敷料仍然是一个巨大的挑战。在此，我们轻松制备了一系列具有固有抗氧化、杀菌和粘附特性的（-）-表没食子儿茶素-3-没食子酸酯（EGCG）交联的羧甲基壳聚糖基水凝胶（EP凝胶）。将葡萄糖酸封端的聚乙二醇（PEG-葡萄糖）引入凝胶网络以增强机械性能。水凝胶是通过PBA接枝的羧甲基壳聚糖（CMCS-PBA）的苯硼酸（PBA）基团与EGCG和PEG-葡萄糖的二醇基团之间的硼酸酯交联构建的。这些水凝胶表现出优异的自愈性能、理想的机械和粘附强度、自由基清除能力以及对[具体细菌1]和[具体细菌2]的出色杀菌能力。在随后的小鼠全层皮肤缺损模型中，EP1凝胶可以促进增殖和重塑过程，如表皮、真皮和皮肤附属器的再生、胶原蛋白的沉积以及VEGF水平的上调，从而加速受损皮肤愈合。总体而言，我们轻松制备了具有固有理想特性的多糖基水凝胶，作为有前景的伤口修复敷料。
