Laboratorio RAMSES, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.
Clinica Ortopedica e Traumatologica 2, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.
Int J Mol Sci. 2023 Aug 3;24(15):12401. doi: 10.3390/ijms241512401.
Adipose tissue-derived cell-based injectable therapies have been demonstrated to have disease-modifying effects on joint tissues in preclinical studies on animal osteoarthritis (OA) models, but clinical results are heterogeneous and not always satisfactory. The aim of this study was to investigate the influence of adipose tissue properties on the therapeutic effects of the adipose-derived product in an in vitro OA setting. Micro-fragmented adipose tissue (MF-AT) samples were obtained from 21 OA patients (mean age 51.7 ± 11.8 years, mean BMI 25.7 ± 4.1 kg/m). The analysis of the MF-AT supernatant was performed to analyze the release of inflammatory factors. The effects of MF-AT inflammatory factors were investigated on chondrocytes and synoviocytes gene expression levels. Patients' characteristics were analyzed to explore their influence on MF-AT inflammatory molecules and on the MF-AT effects on the gene expression of chondrocytes and synoviocytes. The study results demonstrated that adipose tissue-derived products may present inflammatory properties that influence the therapeutic potential for OA treatment, with products with a higher pro-inflammatory profile stimulating a higher expression of genes related to a more inflamed and catabolic phenotype. A higher pro-inflammatory cytokine pattern and a higher pro-inflammatory effect were found in adipose tissue-derived products obtained from OA patients with higher BMI.
脂肪组织来源的细胞注射治疗已被证明在动物骨关节炎 (OA) 模型的临床前研究中对关节组织具有疾病修饰作用，但临床结果存在异质性，并不总是令人满意。本研究旨在探讨脂肪组织特性对体外 OA 环境下脂肪衍生产品治疗效果的影响。从 21 名 OA 患者（平均年龄 51.7 ± 11.8 岁，平均 BMI 25.7 ± 4.1 kg/m）中获得微粉碎脂肪组织 (MF-AT) 样本。分析 MF-AT 上清液以分析炎症因子的释放。研究了 MF-AT 炎症因子对软骨细胞和滑膜细胞基因表达水平的影响。分析了患者的特征，以探讨它们对 MF-AT 炎症分子和 MF-AT 对软骨细胞和滑膜细胞基因表达的影响。研究结果表明，脂肪组织来源的产品可能具有炎症特性，影响 OA 治疗的治疗潜力，具有更高促炎特征的产品刺激与更炎症和分解代谢表型相关的基因更高表达。在 BMI 较高的 OA 患者中获得的脂肪组织衍生产品中发现了更高的促炎细胞因子模式和更高的促炎作用。
