聚脱氧核苷酸和脂肪组织来源的间充质干细胞在犬骨关节炎细胞模型中的抗炎作用。

Anti-inflammatory effects of polydeoxyribonucleotide and adipose tissue-derived mesenchymal stem cells in a canine cell model of osteoarthritis.

机构信息

Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.

BK21 FOUR Future Veterinary Medicine Leading Education and Research Center, Seoul National University, Seoul 08826, Korea.

出版信息

J Vet Sci. 2024 Sep;25(5):e68. doi: 10.4142/jvs.24147.

DOI:10.4142/jvs.24147

PMID:39363656

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11450397/

Abstract

IMPORTANCE

A relatively new therapeutic agent for osteoarthritis (OA), polydeoxyribonucleotide (PDRN), shows potential in treating human OA due to its regenerative and anti-inflammatory effects. However, studies on PDRN for canine OA are limited, and no study has investigated their use with mesenchymal stem cells (MSCs) conventionally used for OA treatment.

OBJECTIVE

This study aimed to evaluate the potential of PDRN and explore its combined effect with adipose tissue-derived MSCs (AdMSCs) in treating canine OA.

METHODS

To study the impact of PDRN, canine chondrocytes, synoviocytes, and AdMSCs were exposed to various PDRN concentrations, and viability was assessed using cell counting kit-8. The OA model was created by treating chondrocytes and synoviocytes with lipopolysaccharide, followed by treatment under three different conditions: PDRN alone, AdMSCs alone, and a combination of PDRN and AdMSCs. Using real-time quantitative polymerase chain reaction, the anti-inflammatory effects and mechanisms were investigated by quantitatively assessing pro-inflammatory cytokines, collagen degradation markers, adenosine A2a receptor (ADORA2A), and nuclear factor-kappa B.

RESULTS

PDRN alone and combined with AdMSCs significantly reduced the expression of pro-inflammatory cytokines and collagen degradation markers in an OA model. PDRN promoted AdMSC proliferation and upregulated ADORA2A expression. AdMSCs exhibited comprehensive anti-inflammatory effects through paracrine effects, and both substances reduced inflammatory gene expression through different mechanisms, potentially enhancing therapeutic effects.

CONCLUSIONS AND RELEVANCE

The results indicate that PDRN is a safe and effective anti-inflammatory material that can be used independently or as an adjuvant for AdMSCs. Although additional research is necessary, this study is significant because it provides a foundation for future research at the cellular level.

摘要

重要性

多聚脱氧核苷酸（PDRN）是一种治疗骨关节炎（OA）的新型治疗药物，由于其再生和抗炎作用，在治疗人类 OA 方面具有潜在作用。然而，关于犬 OA 的 PDRN 研究有限，并且没有研究调查其与间充质干细胞（MSCs）联合使用，这些干细胞通常用于 OA 治疗。

目的

本研究旨在评估 PDRN 的潜力，并探索其与传统用于 OA 治疗的脂肪组织源性 MSCs（AdMSCs）联合使用的效果。

方法

为了研究 PDRN 的影响，用不同浓度的 PDRN 处理犬软骨细胞、滑膜细胞和 AdMSCs，使用细胞计数试剂盒-8 评估细胞活力。用脂多糖处理软骨细胞和滑膜细胞来建立 OA 模型，然后在三种不同条件下进行治疗：单独使用 PDRN、单独使用 AdMSCs、PDRN 和 AdMSCs 联合使用。通过实时定量聚合酶链反应，定量评估促炎细胞因子、胶原降解标志物、腺苷 A2a 受体（ADORA2A）和核因子-κB，研究抗炎效果和机制。