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从 的水提取物制备的生物合成银纳米聚集体对 种细菌的生物活性

Biological Activity of Biosynthesized Silver Nanoaggregates Prepared from the Aqueous Extract of against spp.

作者信息

Al-Otibi Fatimah, Albulayhid Luluwah S, Alharbi Raedah I, Almohsen Atheer A, AlShowiman Ghada M

机构信息

Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11495, Saudi Arabia.

出版信息

Nanomaterials (Basel). 2023 Jul 28;13(15):2198. doi: 10.3390/nano13152198.

Abstract

is commonly used in folk medicine for the treatment of nervous and gastrointestinal disturbances and other medical issues because of its potent antioxidant capacity. The current study evaluated the anti-candida effects of silver nanoparticles (AgNPs) synthesized from an aqueous extract of against different spp. The aqueous extract was prepared from the fresh leaves of . The silver nanoparticles (AgNPs) were prepared and validated by UV spectroscopy, Fourier-transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), and zeta size analysis. , , , , , , and were used in the antifungal assay. Microscopical imaging were used to investigate the different morphological changes induced by treatment. FTIR spectrum confirmed the existence of various functional groups of biomolecules capping the nanoparticles. The average particle size of synthesized AgNPs was 100.6 nm by zeta-sizer and 0.012 to 0.059 mm by TEM. In the antifungal assay, AgNPs aggregates induced significant inhibition of the growth of all species ( < 0.05) compared to the control and the biofilm maturation in and . These considerable antifungal activities might lead to the development of appropriate alternative remedy for the treatment of fungal infections.

摘要

由于其强大的抗氧化能力,它在民间医学中常用于治疗神经和胃肠道紊乱以及其他医学问题。当前的研究评估了由[植物名称]水提取物合成的银纳米颗粒(AgNPs)对不同[念珠菌种类]的抗念珠菌作用。水提取物由[植物名称]的新鲜叶子制备。银纳米颗粒(AgNPs)通过紫外光谱、傅里叶变换红外光谱(FTIR)、透射电子显微镜(TEM)和zeta尺寸分析进行制备和验证。[念珠菌种类1]、[念珠菌种类2]、[念珠菌种类3]、[念珠菌种类4]、[念珠菌种类5]、[念珠菌种类6]和[念珠菌种类7]用于抗真菌试验。显微镜成像用于研究处理诱导的不同形态变化。FTIR光谱证实了包覆纳米颗粒的生物分子各种官能团的存在。通过zeta粒度分析仪测得合成的AgNPs平均粒径为100.6 nm,通过TEM测得为0.012至0.059 mm。在抗真菌试验中,与对照相比,AgNPs聚集体对所有菌种的生长均有显著抑制作用(P<0.05),并且对[念珠菌种类1]和[念珠菌种类2]中的生物膜成熟也有抑制作用。这些显著的抗真菌活性可能会导致开发出治疗真菌感染的合适替代疗法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e4/10421022/8cbf0c65f3ac/nanomaterials-13-02198-g001.jpg

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