College of Mechanical, Naval Architecture & Ocean Engineering, Beibu Gulf University, Qinzhou 535011, China.
Eastern Michigan Associated Engineering College, Beibu Gulf University, Qinzhou 535011, China.
Molecules. 2023 Jul 27;28(15):5701. doi: 10.3390/molecules28155701.
Phenanthrene (PHE), as a structurally simple, tricyclic, polycyclic aromatic hydrocarbon (PAHs), is widely present in marine environments and organisms, with serious ecological and health impacts. It is crucial to study fast and simple high-sensitivity detection methods for phenanthrene in seawater for the environment and the human body. In this paper, a immunosensor was prepared by using a multi-wall carbon nanotube (MWCNTs)-chitosan oligosaccharide (COS) nanocomposite membrane loaded with phenanthrene antibody. The principle was based on the antibody-antigen reaction in the immune reaction, using the strong electron transfer ability of multi-walled carbon nanotubes, coupled with chitosan oligosaccharides with an excellent film formation and biocompatibility, to amplify the detection signal. The content of the phenanthrene in seawater was studied via differential pulse voltammetry (DPV) using a potassium ferricyanide system as a redox probe. The antibody concentration, pH value, and probe concentration were optimized. Under the optimal experimental conditions, the response peak current of the phenanthrene was inversely proportional to the concentration of phenanthrene, in the range from 0.5 ng·mL to 80 ng·mL, and the detection limit was 0.30 ng·mL. The immune sensor was successfully applied to the detection of phenanthrene in marine water, with a recovery rate of 96.1~101.5%, and provided a stable, sensitive, and accurate method for the real-time monitoring of marine environments.
菲（PHE）作为一种结构简单的三环、多环芳烃（PAHs），广泛存在于海洋环境和生物中，对生态和健康有严重影响。因此，研究海水中菲的快速、简单的高灵敏度检测方法对于环境和人体健康至关重要。本文采用多壁碳纳米管（MWCNTs）-壳聚糖寡糖（COS）纳米复合膜负载菲抗体，制备了一种免疫传感器。其原理基于免疫反应中的抗体-抗原反应，利用多壁碳纳米管的强电子转移能力，结合具有良好成膜性和生物相容性的壳聚糖寡糖，对检测信号进行放大。采用以铁氰化钾体系为氧化还原探针的差分脉冲伏安法（DPV）研究海水中菲的含量。优化了抗体浓度、pH 值和探针浓度。在最佳实验条件下，菲的响应峰电流与菲的浓度呈反比，在 0.5ng·mL至 80ng·mL范围内，检测限为 0.30ng·mL。该免疫传感器成功应用于海洋水中菲的检测，回收率为 96.1%至 101.5%，为海洋环境的实时监测提供了一种稳定、灵敏、准确的方法。
