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用于植物遗传资源下一代研究的RDA基因库与数字表型分析

RDA-Genebank and Digital Phenotyping for Next-Generation Research on Plant Genetic Resources.

作者信息

Kim Seong-Hoon, Subramanian Parthiban, Na Young-Wang, Hahn Bum-Soo, Kim Yoonha

机构信息

National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 5487, Republic of Korea.

Laboratory of Crop Production, Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.

出版信息

Plants (Basel). 2023 Jul 31;12(15):2825. doi: 10.3390/plants12152825.

Abstract

The National Agrobiodiversity Center under the Rural Development Administration (RDA) in Jeonju, Republic of Korea stands as the foremost national genebank in the country. Over the years, the National Agrobiodiversity Center has remained committed to enriching its collection with foreign genetic resources, elevating its status to a world-class plant genetic resources (PGR)- holding genebank. Currently, several steps are being undertaken to improve the accessibility of the collection to national as well as international researchers, improve the data available on the resources, and amend the passport information for the accessions. With the implementation of the Nagoya Protocol, the origin of genetic resources is being highlighted as an important input in the passport information. The RDA-Genebank actively responds to the Nagoya Protocol by supplementing passport data for resources lacking information on their origin. In addition, a large number of conserved resources are continuously multiplied, and agronomic traits are investigated concurrently. With the traditional methods of characterization of the germplasm requiring a significant amount of time and effort, we have initiated high-throughput phenotyping using digital techniques to improve our germplasm data. Primarily, we have started adding seed phenotype information followed by measuring root phenotypes which are stored under agronomic traits. This may be the initial step toward using largescale high-throughput techniques for a germplasm. In this study, we aim to provide an introduction to the RDA-Genebank, to adopted international standards, and to the establishment of high-throughput phenotyping techniques for the improvement of passport information.

摘要

韩国全罗北道农村振兴厅下属的国家农业生物多样性中心是该国最重要的国家基因库。多年来,国家农业生物多样性中心一直致力于通过引进国外遗传资源来丰富其种质库,使其地位提升为世界级的植物遗传资源保存基因库。目前,正在采取多项措施,以提高国家和国际研究人员对种质库的访问便利性,改善资源相关数据,并修订种质的护照信息。随着《名古屋议定书》的实施,遗传资源的来源被视为护照信息中的一项重要内容。农村振兴厅基因库通过补充缺乏来源信息的资源的护照数据,积极响应《名古屋议定书》。此外,大量保存的资源在不断繁殖的同时,还会同步研究其农艺性状。由于传统的种质特征鉴定方法需要大量时间和精力,我们已开始采用数字技术进行高通量表型分析,以完善我们的种质数据。首先,我们已开始添加种子表型信息,随后测量根系表型,并将其存储在农艺性状项下。这可能是朝着对种质采用大规模高通量技术迈出的第一步。在本研究中,我们旨在介绍农村振兴厅基因库、所采用的国际标准以及为完善护照信息而建立的高通量表型分析技术。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e9e/10421229/6cc4305acd4d/plants-12-02825-g001.jpg

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