Patlay Aleksandra A, Belousov Andrei S, Silant'ev Vladimir E, Shatilov Roman A, Shmelev Mikhail E, Kovalev Valeri V, Perminova Irina V, Baklanov Ivan N, Kumeiko Vadim V
Institute of Life Sciences and Biomedicine, Far Eastern Federal University, Vladivostok 690922, Russia.
Laboratory of Electrochemical Processes, Institute of Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok 690022, Russia.
Polymers (Basel). 2023 Aug 2;15(15):3280. doi: 10.3390/polym15153280.
Prospective adjuvant anticancer therapy development includes the establishing of drug delivery systems based on biocompatible and biodegradable carriers. We have designed films and nanoparticles (NPs) based on low-esterified pectin hydrogel using the ionic gelation method. We investigated morphology, nanomechanical properties, biocompatibility and anticancer activity. Hydrogel films are characterized by tunable viscoelastic properties and surface nanoarchitectonics through pectin concentration and esterification degree (DE), expressed in variable pore frequency and diameter. An in vitro study showed a significant reduction in metabolic activity and the proliferation of the U87MG human glioblastoma cell line, probably affected via the adhesion mechanism. Glioma cells formed neurosphere-like conglomerates with a small number of neurites when cultured on fully de-esterified pectin films and they did not produce neurites on the films prepared on 50% esterified pectin. Pectin NPs were examined in terms of size distribution and nanomechanical properties. The NPs' shapes were proved spherical with a mean diameter varying in the range of 90-115 nm, and a negative zeta potential from -8.30 to -7.86 mV, which indicated their stability. The NPs did not demonstrate toxic effect on cells or metabolism inhibition, indicating good biocompatibility. Nanostructured biomaterials prepared on low-esterified pectins could be of interest for biomedical applications in adjuvant anticancer therapy and for designing drug delivery systems.
前瞻性辅助抗癌治疗的发展包括基于生物相容性和可生物降解载体建立药物递送系统。我们采用离子凝胶法设计了基于低酯化果胶水凝胶的薄膜和纳米颗粒（NPs）。我们研究了其形态、纳米力学性能、生物相容性和抗癌活性。水凝胶薄膜的特点是通过果胶浓度和酯化度（DE）来调节粘弹性性能和表面纳米结构，表现为可变的孔隙频率和直径。体外研究表明，U87MG人胶质母细胞瘤细胞系的代谢活性和增殖显著降低，可能是通过粘附机制受到影响。当在完全脱酯化的果胶薄膜上培养时，胶质瘤细胞形成了带有少量神经突的神经球样聚集体，而在50%酯化果胶制备的薄膜上它们不产生神经突。对果胶纳米颗粒的尺寸分布和纳米力学性能进行了研究。结果证明纳米颗粒呈球形，平均直径在90-115nm范围内，zeta电位为-8.30至-7.86mV，表明其稳定性。纳米颗粒对细胞未表现出毒性作用或代谢抑制作用，表明具有良好的生物相容性。基于低酯化果胶制备的纳米结构生物材料可能在辅助抗癌治疗的生物医学应用和药物递送系统设计方面具有应用价值。
