联合蛋白质组学和转录组学鉴定出丝氨酸蛋白酶抑制剂家族 C 成员 1 相关蛋白作为子宫内膜异位症的生物标志物。
Combined proteomics and transcriptomics identifies serpin family C member 1 associated protein as a biomarker of endometriosis.
机构信息
Department of Obstetrics and Gynecology, National Clinical Research Center for Obstetric & Gynecologic Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China.
Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, PR China.
出版信息
Ann Med. 2023;55(2):2243825. doi: 10.1080/07853890.2023.2243825.
OBJECTIVE
To explore potential biomarkers indicating endometriosis (EM).
MATERIALS AND METHODS
A proteomics method and combined quantitative transcriptomics were adopted to highlight markers in the EM. Venn analysis was used to integrate the ribonucleic acid sequencing (RNA-seq) and protein profiles. Promising candidate markers were tested by enzyme-related immunosorbent assay.
RESULTS
A sum of 979 mRNAs and 39 proteins were tested to be significantly differentially expression in the standard cluster compared with the EM cluster. Venn analysis showed a filtered signature of only two down-regulated molecules in the EM group, i.e. fetuin B (FETUB) and serpin family C member 1 (SERPINC1); the latter showed a big variance between the control category and the EM set in the authentication test.
CONCLUSION
SERPINC1 may be a useful possible biomarker for the analysis of EM.
目的
探索子宫内膜异位症（EM）相关的潜在生物标志物。
材料与方法
采用蛋白质组学方法和联合定量转录组学，以突出 EM 中的标志物。采用 Venn 分析整合 RNA 测序（RNA-seq）和蛋白图谱。通过酶相关免疫吸附测定法对有前途的候选标志物进行测试。
结果
在标准簇与 EM 簇相比，共检测到 979 个 mRNA 和 39 个蛋白质的显著差异表达。Venn 分析显示，EM 组中仅有两个下调分子的过滤特征，即胎球蛋白 B（FETUB）和丝氨酸蛋白酶家族 C 成员 1（SERPINC1）；在后一种情况下，在验证测试中，SERPINC1 在对照组和 EM 组之间的差异很大。
结论
SERPINC1 可能是分析 EM 的有用潜在生物标志物。
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