Seshadri V Ranjith Akshay, Varghese Nikitha S, Gurunathan Deepa
Pediatric and Preventive Dentistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, IND.
Cureus. 2023 Jul 11;15(7):e41735. doi: 10.7759/cureus.41735. eCollection 2023 Jul.
Introduction Untreated dental decay poses a significant oral health challenge, leading to pain, tooth loss, and infections. Fluoride varnishes are in prolonged contact with the tooth surface and this prevents dental decay. However, limited research has been conducted regarding the cytotoxicity and cytocompatibility of varnishes on oral cells. Recent studies have shed light on the cytotoxic effect of these varnishes on human fibroblast cells. Material and Methods The fibroblasts were isolated and cultured in 0.00001, 0.0001, 0.001, 0.01, 0.1, and 1 % fluoride concentration The cells were incubated for 72 hours at a temperature of 37°C and cell viability after the application of varnish was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay. Results This study observed that fluoride varnish had a concentration dependant cytotoxic effect on human gingival fibroblasts (hGFs). As the concentration of fluoride increased, the cell viability decreased. At 1% concentration, there was maximum cell cytotoxicity. At the lowest concentration (0.00001), more than 78% of the cells were found to be viable. Conclusion Further research is necessary to develop safer and more biocompatible fluoride varnish formulations to ensure their efficacy in preventing dental caries without causing harm to oral tissues.
引言 未经治疗的龋齿对口腔健康构成重大挑战，会导致疼痛、牙齿脱落和感染。氟化物清漆与牙齿表面长时间接触，可预防龋齿。然而，关于清漆对口腔细胞的细胞毒性和细胞相容性的研究有限。最近的研究揭示了这些清漆对人成纤维细胞的细胞毒性作用。
材料与方法 将成纤维细胞分离并在0.00001%、0.0001%、0.001%、0.01%、0.1%和1%的氟化物浓度下培养。细胞在37°C下孵育72小时，使用3-(4,5-二甲基噻唑-2-基)-二苯基溴化四氮唑(MTT)法评估涂覆清漆后的细胞活力。
结果 本研究观察到氟化物清漆对人牙龈成纤维细胞(hGFs)具有浓度依赖性细胞毒性作用。随着氟化物浓度的增加，细胞活力下降。在1%的浓度下，细胞毒性最大。在最低浓度(0.00001%)时，发现超过78%的细胞具有活力。
结论 有必要进行进一步研究，以开发更安全、生物相容性更好的氟化物清漆配方，确保其在预防龋齿的同时不会对口腔组织造成损害。
