Parirokh Masoud, Forghani Farshid Reza, Paseban Hamzeh, Asgary Saeed, Askarifard Sara, Esmaeeli Mahani Saeed
Kerman Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran;
Oral and Dental Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran;
Iran Endod J. 2015;10(2):89-92. Epub 2015 Mar 18.
Assessment of cellular cytotoxicity is a regular method for evaluating the biocompatibility of novel materials. In a recent study, 5% fluoride varnish (Duraflur) has shown reasonable sealing ability and coverage of root canal walls when used as a sealer. The aim of the present study was to compare the cytotoxicity of Duraflur varnish with two popular commonly used root canal sealers (AH-Plus and AH-26) on human gingival fibroblasts (HGF).
The HGFs were incubated with different concentrations (1/2, 1/4, and 1/8) of AH-plus, AH-26, and Duraflur varnish for 24 h. The percentage of cell viability was assessed with methyl-thiazol-tetrazolium (MTT) assay. The data was analyzed using the one-way ANOVA followed by Student-Newman-Keuls test. The level of significance was set at 0.001.
MTT assay showed that higher concentrations of the tested materials resulted in lower viability of HGFs. AH-Plus showed significantly greater cell viability compared to AH-26 at all dilutions (P<0.001); however, no significant difference was found between Duraflur and AH-Plus in terms of cell viability at 1/8 dilution (P>0.001). Duraflur showed significantly higher cell viability compared to AH-26 except at 1/2 dilution (P<0.001).
Although Duraflur varnish had better biocompatibility compared to AH-26, it should still be evaluated with further biocompatibility tests such as intraosseous and subcutaneous implantation.
细胞毒性评估是评估新型材料生物相容性的常规方法。在最近的一项研究中，5%的含氟涂料（Duraphat）用作封闭剂时，显示出合理的封闭能力和对根管壁的覆盖效果。本研究的目的是比较Duraphat含氟涂料与两种常用的根管封闭剂（AH-Plus和AH-26）对人牙龈成纤维细胞（HGF）的细胞毒性。
将HGF与不同浓度（1/2、1/4和1/8）的AH-Plus、AH-26和Duraphat含氟涂料孵育24小时。用甲基噻唑基四唑（MTT）法评估细胞活力百分比。数据采用单因素方差分析，随后进行Student-Newman-Keuls检验。显著性水平设定为0.001。
MTT法显示，测试材料浓度越高，HGF的活力越低。在所有稀释度下，AH-Plus的细胞活力均显著高于AH-26（P<0.001）；然而，在1/8稀释度下，Duraphat和AH-Plus在细胞活力方面未发现显著差异（P>0.001）。除1/2稀释度外，Duraphat的细胞活力显著高于AH-26（P<0.001）。
尽管Duraphat含氟涂料与AH-26相比具有更好的生物相容性，但仍应通过进一步的生物相容性测试，如骨内和皮下植入来进行评估。
