Hao Ke-Xin, Xie Hong, Jiang Jian-Guo, Wang Dawei, Zhu Wei
College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China.
The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
ACS Omega. 2023 Jul 26;8(31):28080-28089. doi: 10.1021/acsomega.3c00433. eCollection 2023 Aug 8.
The role of Semen saponins in sedative and hypnosis has attracted much attention. The study aimed to investigate its possible UV damage protection and anti-aging effects. Total saponins (SZR I) and purified saponins (SZR II) were analyzed and compared by infrared spectroscopy and high-performance liquid chromatography (HPLC). The protective effects of SZR I, SZR II, and their three monomers on HaCaT cells damaged by UV were studied, and their anti-aging activities were observed by with paraquat-induced oxidative stress. The results showed that SZR I and SZR II differ in chemical composition but both have the same three monomers. The cell survival rate treated with SZR I and SZR II at a concentration of 400 μg/mL increased by 34.45 and 88.98%, respectively, indicating that they could promote the proliferation of UVB-damaged HaCaT cells. Jujuboside A, Jujuboside B, and spinosin from the saponins exhibited similar effects on UVB-damaged HaCaT cells. SZR I and SZR II had little effect on reproductive performance but could delay the senescence caused by heat and oxidative stress of the model. These results provide useful data that Semen saponin is a potential natural product with UV damage protection and anti-aging characteristics.
菟丝子总皂苷在镇静催眠方面的作用备受关注。本研究旨在探讨其可能的紫外线损伤防护及抗衰老作用。采用红外光谱法和高效液相色谱法（HPLC）对总皂苷（SZR I）和纯化皂苷（SZR II）进行分析比较。研究了SZR I、SZR II及其三种单体对紫外线损伤的HaCaT细胞的保护作用，并通过百草枯诱导的氧化应激观察其抗衰老活性。结果表明，SZR I和SZR II化学成分不同，但都含有相同的三种单体。浓度为400μg/mL的SZR I和SZR II处理后，细胞存活率分别提高了34.45%和88.98%，表明它们能促进紫外线B损伤的HaCaT细胞增殖。皂苷中的酸枣仁皂苷A、酸枣仁皂苷B和斯皮诺素对紫外线B损伤的HaCaT细胞表现出相似的作用。SZR I和SZR II对生殖性能影响较小，但能延缓该模型因热应激和氧化应激引起的衰老。这些结果为菟丝子总皂苷是一种具有紫外线损伤防护和抗衰老特性的潜在天然产物提供了有用的数据。
