School of Medicine, Hunan Normal University, Changsha, 410013, PR China.
College of Biology, Hunan University, Changsha, 410082, PR China.
Biomater Sci. 2023 Sep 12;11(18):6342-6356. doi: 10.1039/d3bm01039b.
Antibiotic therapy can induce the generation of severe bacterial resistance, further challenging the usability of currently available drugs and treatment options. Therefore, it is essential to develop new strategies to effectively eradicate drug-resistant bacteria. Herein, we have reported a combinational strategy for the eradication of drug-resistant bacteria by using chlorin e6 (Ce6) loaded Prussian blue nanoparticles (PB NPs). This nanocomplex showed strong catalase activity and photodynamic properties. experiments demonstrated that CPB-Ce6 NPs effectively kill MRSA by generating ROS under laser irradiation. Meanwhile, the nano-enzyme activity of CPB NPs can decompose HO in the bacterial microenvironment to upregulate the O level, which in turn alleviates hypoxia in the microenvironment and improves the antibacterial effect of PDT. results demonstrated that CPB-Ce6 NPs with laser irradiation effectively cleared MRSA and promoted infected wound repair in a diabetic mouse model and normal mice through upregulating VEGF. Moreover, CPB-Ce6 NPs showed excellent biosafety profiles and . From our point of view, this PDT based on PB NPs with nano-enzyme activity may provide an effective treatment for infections associated with drug-resistant microbes and tissue repair.
抗生素治疗会导致严重的细菌耐药性产生，进一步挑战了现有药物和治疗方案的可用性。因此，开发新的策略来有效清除耐药菌至关重要。在此，我们报道了一种联合策略，使用负载氯己定（Ce6）的普鲁士蓝纳米颗粒（PB NPs）来清除耐药菌。该纳米复合物表现出很强的过氧化氢酶活性和光动力特性。实验表明，CPB-Ce6 NPs 在激光照射下通过产生 ROS 有效杀死耐甲氧西林金黄色葡萄球菌（MRSA）。同时，CPB NPs 的纳米酶活性可以分解细菌微环境中的 HO，上调 O 水平，从而减轻微环境中的缺氧，并提高 PDT 的抗菌效果。结果表明，CPB-Ce6 NPs 联合激光照射通过上调 VEGF 可有效清除 MRSA，并促进糖尿病小鼠模型和正常小鼠感染伤口的修复。此外，CPB-Ce6 NPs 具有良好的生物安全性。从我们的角度来看，这种基于具有纳米酶活性的 PB NPs 的 PDT 可能为与耐药微生物相关的感染和组织修复提供有效的治疗方法。
