Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran 1439817435, Iran.
Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran 1439817435, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15;303:123234. doi: 10.1016/j.saa.2023.123234. Epub 2023 Aug 4.
In this study, an aptamer-based, functionalized-DNA hydrogel system is developed for prostate-specific antigen (PSA) detection. A pure DNA hydrogel is constructed using specific DNA building blocks and an aptamer as a cross-linker. Firstly, silver nanoclusters (AgNCs) are constructed on the Y-shaped DNA (Y-DNA) building blocks. Then, the DNA hydrogel was formed via the addition of the cross-linker to the Y-DNA solution. In this case, the fluorescence emission of silver nanoclusters that have accumulated in the hydrogel increases due to aggregation-induced emission (AIE). The presence of PSA and its subsequent interaction with its specific aptamer dissolve the hydrogel structures, which leads to a low emission intensity. A great linear relationship was attained in this assay in the range of 0.05 to 8 ng mL with a detection limit of 4.4 pg mL for the detection of PSA. Additionally, the proposed aptasensor was successfully used to detect PSA in human serum samples. The recovery for different concentrations of PSA was in the range of 96.1% to 99.3%, and the RSD range was from 2.3% to 4.5%. Comparing our method to current ones in the field of PSA detection proves that our platform benefits from a simpler procedure, lower cost, and better efficiency, providing high potential for future clinical applications.
在这项研究中，开发了一种基于适体的功能化 DNA 水凝胶系统，用于检测前列腺特异性抗原（PSA）。使用特定的 DNA 构建块和适体作为交联剂构建纯 DNA 水凝胶。首先，在 Y 型 DNA（Y-DNA）构建块上构建银纳米簇（AgNCs）。然后，通过将交联剂添加到 Y-DNA 溶液中来形成 DNA 水凝胶。在这种情况下，由于聚集诱导发射（AIE），聚集在水凝胶中的银纳米簇的荧光发射增加。PSA 的存在及其与特异性适体的随后相互作用会溶解水凝胶结构，从而导致发射强度降低。在该测定中，在 0.05 至 8 ng mL 的范围内获得了很好的线性关系，检测 PSA 的检测限为 4.4 pg mL。此外，该方法成功地用于检测人血清样品中的 PSA。不同浓度 PSA 的回收率在 96.1%至 99.3%之间，RSD 范围为 2.3%至 4.5%。将我们的方法与 PSA 检测领域的当前方法进行比较证明，我们的平台具有更简单的程序、更低的成本和更高的效率，为未来的临床应用提供了很高的潜力。
